MAB1569
Anti-Microglia Antibody, clone 25F9
clone 25F9, Chemicon®, from mouse
Synonym(s):
Macrophages, Late State Inflammatory
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
25F9, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
unmodified
General description
ANTIGEN DISTRIBUTION:
Isolated cells: Absent on freshly isolated monocytes and other blood cells; present on 40-50% of human monocytes after 6-7 day culture, also positive on some melanoma and carcinoma cell lines. Tissue sections: Kupffer cells, histiocytes (skin), macrophages of the thymus, in the germinal centers of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer; excema, sarcoidosis, BCG granuloma; synovial lining cells, tuberculoid leprosy; no expression in lepramatous leprosy.
Isolated cells: Absent on freshly isolated monocytes and other blood cells; present on 40-50% of human monocytes after 6-7 day culture, also positive on some melanoma and carcinoma cell lines. Tissue sections: Kupffer cells, histiocytes (skin), macrophages of the thymus, in the germinal centers of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer; excema, sarcoidosis, BCG granuloma; synovial lining cells, tuberculoid leprosy; no expression in lepramatous leprosy.
Specificity
Human late stage inflammatory macrophages. The monoclonal is suitable for use on BAL (bronchial lavage fluids) and other lavages. The antigen recognized by MAB1569 is an 86 kDa protein (unreduced conditions), probably a glycoprotein on the cell surface and within the cytoplasm of mature macrophages. It is stable to formaldehyde fixation and paraffin embedding. Enzyme digestion is recommended. The antibody has also been reported to recognize microglia stem cells (X International Congress of Neuropathology, Stockholm, le 11 September,1986.PosterA-701).
Antigen distribution: absent from freshly isolated monocytes and other blood cells; present on 40-50% of human monocytes after 6-7 days in culture, also positive on some melanoma and carcinoma lines. In tissue sections, clone identifies Kupffer cells, histiocytes (skin), macrophages of the thymus, in te germinal centers of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer; excema, sarcoidosis, BCG granuloma;synovial lining cells, tuberculoid leprosy, however no expression in lepramatous leprosy.
Species reactivity is seen in human, rhesus monkey, and pig, other species not tested.
Antigen distribution: absent from freshly isolated monocytes and other blood cells; present on 40-50% of human monocytes after 6-7 days in culture, also positive on some melanoma and carcinoma lines. In tissue sections, clone identifies Kupffer cells, histiocytes (skin), macrophages of the thymus, in te germinal centers of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer; excema, sarcoidosis, BCG granuloma;synovial lining cells, tuberculoid leprosy, however no expression in lepramatous leprosy.
Species reactivity is seen in human, rhesus monkey, and pig, other species not tested.
Application
Anti-Microglia Antibody, clone 25F9 detects level of Microglia & has been published & validated for use in FC, IC, IH.
Immunohistochemistry: 1:50-1:200 using human tonsil cryosection. The antibody is reactive on formalin fixed / paraffin sections. Enzyme digestion (proteinase pretreatement) is recommended.
Immunocytochemistry
Optimal working dilutions must be determined by the end user.
Immunocytochemistry
Optimal working dilutions must be determined by the end user.
Physical form
Format: Purified
Purified immunoglobulin from culture supernatant. Lyophilized from PBS, pH 7.2 containing 5 mg/mL BSA and 0.09% sodium azide. Reconstitute with 500 μL of sterile distilled water.
Storage and Stability
Maintain lyophilized material at 2-8°C for up to 6 months. After reconstitution maintain at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Pathogenicity of HIV in lymphatic organs of patients with AIDS.
Pekovic, D D, et al.
The Journal of Pathology, 152, 31-35 (1987)
Infiltration of primary and metastatic melanomas with macrophages of the 25F9-positive phenotype.
Brocker, E B, et al.
Cancer Immunology, Immunotherapy, 25, 81-86 (1987)
Johannes Vogt et al.
EMBO molecular medicine, 8(1), 25-38 (2015-12-17)
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