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FCABS301A4

Sigma-Aldrich

Milli-Mark® Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor™488 Antibody

Milli-Mark®, from rabbit

Synonym(s):

H3 histone family, member T, histone 3, H3, histone cluster 3, H3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG

NCBI accession no.

NP_003484

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

dimethylation (Lys9)

Gene Information

human ... H3C1(8350)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

Antibody recognizes human Histone H3 dimethylated on lysine 9.

Immunogen

Epitope: Methylated at Lys9dimethylated Lys 9 of Human Histone H3
KLH-conjugated, 2X-branched synthetic peptide corresponding to dimethyl-lysine at residue 9 of human Histone H3.

Application

Milli-Mark Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor488 Antibody detects level of dimethyl-Histone H3 (Lys9)-Alexa Fluor488 & has been published & validated for use in FC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Evaluated by flow cytometry using HeLa cells

Target description

15.5 kDa Calculated

Physical form

Protein A purified
Purified rabbit polyclonal IgG conjugated to Alexa Fluor 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA

Storage and Stability

Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.

Analysis Note

Control
HeLa Cells

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Valeria Barili et al.
Nature communications, 11(1), 604-604 (2020-02-01)
Hepatitis C virus infection (HCV) represents a unique model to characterize, from early to late stages of infection, the T cell differentiation process leading to exhaustion of human CD8+ T cells. Here we show that in early HCV infection, exhaustion-committed

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