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CBA059

Sigma-Aldrich

Annexin V-FITC Apoptosis Detection Kit II

fluorometric assay kit for measuring Annexin V in apoptotic cells.

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About This Item

Quality Level

usage

sufficient for 20 tests

packaging

pkg of 1 kit

manufacturer/tradename

Calbiochem®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable

detection method

fluorometric

shipped in

wet ice

storage temp.

2-8°C

General description

A convenient kit for the identification of changes in the plasma membrane that occur during apoptosis. In apoptotic cells, phosphoatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, allowing for the detection of PS on the cell surface. In the presence of Ca 2+, Annexin V exhibits a high affinity for PS and binds cells with exposed PS. Annexin V, when conjugated to a fluorophore, can be used to monitor apoptopic cells using flow cytometry or immunofluorescence microscopy.

Components

Kit contains:
  • Annexin V-FITC
  • Binding Buffer
  • Propidium Iodide
  • user protocol.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

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