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Key Documents

ABC344

Sigma-Aldrich

Anti-ATG13 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Autophagy-related protein 13

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

species reactivity (predicted by homology)

human (immunogen homology)

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

O75143

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ATG13(9776)
mouse ... Atg13(51897)

General description

Autophagy-related protein 13 (ATG13) is an autophagy factor that is involved in the preservation of cellular nutrients under starvation conditions as well as under normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. Knockdown of ATG13 inhibits autophagosome formation.

Immunogen

KLH-conjugated linear peptide corresponding to human ATG13.

Application

Anti-ATG13 Antibody is a highly specific rabbit polyclonal antibody, that targets ATG13 & has been tested in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected ATG13 in mouse heart tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected ATG13 in mouse heart cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting/Peptide Inhibition Assay in rat heart tissue lysate.

Western Blotting/Peptide Inhibition Assay Analysis: 1 µg/mL of this antibody detected ATG13 in 15 µg of rat heart tissue lysate.

Target description

~49 kDa observed. Uniprot describes 5 isoforms ranging between ~44 kDa and ~60 kDa Uncharacterized bands may appear in some lysate(s).

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat heart tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Christopher J Shoemaker et al.
PLoS biology, 17(4), e2007044-e2007044 (2019-04-02)
The power of forward genetics in yeast is the foundation on which the field of autophagy research firmly stands. Complementary work on autophagy in higher eukaryotes has revealed both the deep conservation of this process, as well as novel mechanisms

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