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Key Documents

AB3152

Sigma-Aldrich

Anti-Amphiregulin Antibody

Chemicon®, from rabbit

Synonym(s):

AR, CRDGF, KAF

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NM_001657.2

UniProt accession no.

P15514

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... AREG(374)

Specificity

Recognizes a glycoprotein of 18.5-22.5kDa, identified as amphiregulin (AR). Antibody is directed against the region of AR, which binds to heparin and is highly specific to AR. It shows no cross-reaction with EGF, TGFalpha, or HB-EGF. In vitro, AR functions as an autocrine growth stimulator to derive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells, and keratinocytes. AR is important in development of human placenta and murine mammary gland.

Immunogen

A synthetic 19-mer peptide, corresponding to aa 26-44 from the secreted amphiregulin of human origin.

Application

Immunoprecipitation (Use Protein A): Ab 10μg/mg protein lysate.

Western Blotting: Ab 5μg/ml for 2hrs at room temperature.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
This Anti-Amphiregulin Antibody is validated for use in ELISA, IP, WB for the detection of Amphiregulin.

Physical form

Format: Purified
Purified immunoglobulin from rabbit anti-serum by Protein A chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Shoyab et al.
Science (New York, N.Y.), 243(4894 Pt 1), 1074-1076 (1989-02-24)
The complete amino acid sequence of amphiregulin, a bifunctional cell growth modulator, was determined. The truncated form contains 78 amino acids, whereas a larger form of amphiregulin contains six additional amino acids at the amino-terminal end. The amino-terminal half of

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