AB16969
Anti-Caspase 9 Antibody, proform & lg. subunit
Chemicon®, from rabbit
Synonym(s):
ICE-LAP6, Mch-6, Apaf-3
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
should not react with
rat
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CASP9(842)
Specificity
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (Duan et al. 1996; Srinivasula et al. 1996; Nijhawan et al. 1997). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (Nijhawan et al. 1997). Caspase-9 is also activated by granzyme B and CPP32 (Duan et al. 1996; Srinivasula et al. 1996). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (Nijhawan et al. 1997). Caspase-9 plays a central role in cell death induced by a wide variety of apoptosis inducers including TNF-alpha, TRAIL, anti-CD-95, FADD, and TRADD (Pan et al. 1998). Caspase-9 is expressed in a variety of human tissues (Duan et al. 1996; Srinivasula et al. 1996).
Immunogen
Epitope: proform & lg. subunit
Synthetic peptide corresponding to amino acids 41-56 of human caspase-9 (Duan et al. 1996; Srinivasula et al. 1996; Nijhawan et al. 1997). Recognizes proform (46 kDa); sequence analysis predicts cross-reactivity with mouse caspase-9 (>90%).
Application
Detect Caspase 9 using this Anti-Caspase 9 Antibody, proform & lg. subunit validated for use in WB.
Research Category
Apoptosis & Cancer
Metabolism
Apoptosis & Cancer
Metabolism
Research Sub Category
Caspases
Enzymes & Biochemistry
Caspases
Enzymes & Biochemistry
Western blot: 1:1,000 to 1:2,000
Whole cell lysate from HeLa cells can be used as a positive control and a 46 kDa band should be detected.
Optimal working dilutions must be determined by end user.
Whole cell lysate from HeLa cells can be used as a positive control and a 46 kDa band should be detected.
Optimal working dilutions must be determined by end user.
Linkage
Replaces: 04-443
Physical form
Format: Purified
Immunoaffinity chromatography purified immunoglobulin. Liquid in PBS, containing 0.02% sodium azide.
Storage and Stability
The undiluted antibody solution is stable for approximately 6 months when stored 2-8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mitochondrial-nuclear communication by prohibitin shuttling under oxidative stress.
Srinivas R Sripathi,Weilue He,Cameron L Atkinson,Joseph J Smith,Zhicong Liu et al.
Biochemistry null
Lack of pathology in a triple transgenic mouse model of Alzheimer's disease after overexpression of the anti-apoptotic protein Bcl-2.
Rohn, TT; Vyas, V; Hernandez-Estrada, T; Nichol, KE; Christie, LA; Head, E
The Journal of Neuroscience null
S M Srinivasula et al.
The Journal of biological chemistry, 275(46), 36152-36157 (2000-08-22)
Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and promotes cytochrome c-dependent caspase activation by neutralizing inhibitor of apoptosis proteins (IAPs). We provide evidence that Smac/DIABLO functions at the levels of both the Apaf-1-caspase-9
G Pan et al.
Science (New York, N.Y.), 276(5309), 111-113 (1997-04-04)
TRAIL (also known as Apo-2L) is a member of the tumor necrosis factor (TNF) ligand family that rapidly induces apoptosis in a variety of transformed cell lines. The human receptor for TRAIL was found to be an undescribed member of
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