AB1557

Anti-NMDAR2B Antibody, pain

serum, Chemicon^®

• Synonym(s): N-methyl D-aspartate Receptor Subtype 2B, NR2B
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, human, rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• suitability: not suitable for immunohistochemistry
• NCBI accession no.: NM_000834.2
• UniProt accession no.: Q13224
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... GRIN2B(2904)

Description

Specificity

Recognizes NMDAR2B. By Western blot it recognizes a 180 kDa band in rat brain membranes. Also recognizes a 70 kDa band (thought to be a contaminant). No reactivity to NMDAR2A or NMDAR2C by Western blot. Immunolabeling is blocked by preadsorption of AB1557 with the C-terminal fusion protein that was used to generate the antibody.

Immunogen

C-Terminal Fusion Protein of the NMDAR2B (Amino acids 984-1104).

Application

Anti-NMDAR2B Antibody, pain detects level of NMDAR2B & has been published & validated for use in IP & WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Western blotting 1:200-1:1,000 (NMDAR2B is present in high concentrations in the hippocampus)


Immunoprecipitation using solubilized hippocampal slices. 3 μL will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200 μg of rat brain lysate.


Not suggested for use in immunohistochemistry.


Note: Above dilutions are with 35S-protein A; with ECL dilutions may need to be considerably higher to obtain specific immunolabeling.


Optimal working dilutions must be determined by the end user.

Target description

180 kDa

Physical form

Rabbit antiSerum. Liquid, no preservatives.

Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain tissue, Rat Purkinje cell dendrites, rat hippocampus lysate, HEK 293 cells expressing NR2B

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable