681673

Wnt Antagonist III, Box5

The Wnt Antagonist III, Box5 controls the biological activity of Wnt. This small molecule/inhibitor is primarily used for Cancer applications.

• Synonym(s): Wnt Antagonist III, Box5, Wnt Pathway Inhibitor VI, Boc-MDGCEL-OH, Frizzled-5 Antagonist, Wnt-5a Antagonist
• Empirical Formula (Hill Notation): C₃₀H₅₀N₆O₁₃S₂
• Molecular Weight: 766.88
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥90% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: white
• solubility: water: 4 mg/mL; DMSO: 50 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A Wnt-5a-derived, N-terminally butyloxycarbonyl- (Boc) protected, hexa-peptide that effectively antagonizes Wnt-5a-mediated cellular activities in A2058 and HTB63 melanoma cultures, including migration, invasion, calcium response, and MARCKS phosphorylation. Replacing the N-terminal Boc with a formyl modification results in a peptide termed Foxy5 that mimics, instead of antagonizes, Wnt-5a biological activity in a Frizzled-5- (Fzd-5/Frz-5) dependent manner. The peptide sequence is conserved in multiple species, including human, mouse, and rat, and Box5 is expected to exert its effect via direct FZD5 binding as is the case with Foxy5.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

t-Boc-NH-Met-Asp-Gly-Cys-Glu-Leu-CO₂H

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Jenei, V., et al. 2009. Proc. Natl. Acad. Sci. USA106, 19473.
Safholm, A., et al. 2006. J. Biol. Chem.281, 2740.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable