5.30650

Luciferase Substrate, CycLuc1

• Synonym(s): Luciferase Substrate, CycLuc1
• Empirical Formula (Hill Notation): C₁₃H₁₁N₃O₂S₂
• Molecular Weight: 305.38
• UNSPSC Code: 12352200

Properties

• Assay: ≥94% (HPLC)
• Quality Level: 100
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: DMSO: 100 mg/mL
• storage temp.: 2-8°C

Description

General description

A synthetic cyclic alkylaminoluciferin that exhibits properties that are superior to those of D-luciferin. Binds to luciferase with higher affinity and and emits about 5.7-fold more light than aminoluciferin and 3.2-fold more light than D-luciferin. Its superior light output is maintained over a wide range (1-100 µM). Shown to readily cross the blood-brain barrier in mice and provides much improved noninvasive bioluminescence imaging (BLI) of the brain at lower dose when compared to D-luciferin. Yields about 10-fold higher bioluminescent signal than D-luciferin when Luc2 luciferase expressing 4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice. A rapid (4-5 min), long-lasting, and steady signal peak is observed following an i.v injection CycLuc1 in mice.

A synthetic cyclic alkylaminoluciferin that exhibits properties that are superior to those of D-luciferin. Binds to luciferase with higher affinity and and emits about 5.7-fold more light than aminoluciferin and 3.2-fold more light than D-luciferin. Its superior light output is maintained over a wide range (1-100 µM). Shown to readily cross the blood-brain barrier in mice and provides much improved noninvasive bioluminescence imaging (BLI) of the brain at lower dose when compared to D-luciferin. Yields about 10-fold higher bioluminescent signal than D-luciferin when Luc2 luciferase expressing 4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice. A rapid (4-5 min), long-lasting, and steady signal peak is observed following an i.v injection CycLuc1 in mice.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Luciferase

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Other Notes

Evans, M., et al. 2014. Nat Methods.11, 393.
Reddy, G. R, et al. 2010. J. Am. Chem. Soc.132, 13586.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable