401002

Iberiotoxin, Buthus tamulus, Recombinant, E. coli

• Synonym(s): Iberiotoxin, Buthus tamulus, Recombinant, E. coli, rlbTx, Pyr-FTDVDCSVSKECWSVCKDLFGVDRGKCMGKKCRCYQ
• CAS Number: 129203-60-7
• MDL number: MFCD00131365
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Assay: ≥98% (HPLC)
• Quality Level: 100
• form: lyophilized solid (Salt-free)
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C179H274N50O55S7/c1-87(2)65-111-155(261)208-112(66-93-33-15-13-16-34-93)146(252)195-77-133(239)225-139(88(3)4)172(278)213-117(71-136(244)245)158(264)199-100(44-31-62-190-178(186)187)144(250)193-75-131(237)198-102(40-22-27-58-181)148(254)219-124(167(273)205-109(56-64-285-12)145(251)194-76-132(238)197-101(39-21-26-57-180)147(253)200-105(43-25-30-61-184)151(257)220-123-81-286-288-83-125(221-152(258)106(203-166(123)272)45-32-63-191-179(188)189)169(275)210-113(68-95-46-48-97(234)49-47-95)156(262)206-110(177(283)284)50-53-129(185)235)82-287-291-86-128(168(274)202-104(42-24-29-60-183)150(256)212-116(70-135(242)243)159(265)207-111)224-175(281)142(91(9)10)228-164(270)121(79-231)216-157(263)115(69-96-74-192-99-38-20-19-37-98(96)99)211-170(276)126-84-289-290-85-127(171(277)217-122(80-232)165(271)227-141(90(7)8)174(280)218-120(78-230)163(269)201-103(41-23-28-59-182)149(255)204-108(154(260)222-126)52-55-134(240)241)223-160(266)118(72-137(246)247)214-173(279)140(89(5)6)226-162(268)119(73-138(248)249)215-176(282)143(92(11)233)229-161(267)114(67-94-35-17-14-18-36-94)209-153(259)107-51-54-130(236)196-107/h13-20,33-38,46-49,74,87-92,100-128,139-143,192,230-234H,21-32,39-45,50-73,75-86,180-184H2,1-12H3,(H2,185,235)(H,193,250)(H,194,251)(H,195,252)(H,196,236)(H,197,238)(H,198,237)(H,199,264)(H,200,253)(H,201,269)(H,202,274)(H,203,272)(H,204,255)(H,205,273)(H,206,262)(H,207,265)(H,208,261)(H,209,259)(H,210,275)(H,211,276)(H,212,256)(H,213,278)(H,214,279)(H,215,282)(H,216,263)(H,217,277)(H,218,280)(H,219,254)(H,220,257)(H,221,258)(H,222,260)(H,223,266)(H,224,281)(H,225,239)(H,226,268)(H,227,271)(H,228,270)(H,229,267)(H,240,241)(H,242,243)(H,244,245)(H,246,247)(H,248,249)(H,283,284)(H4,186,187,190)(H4,188,189,191)
• InChI key: VDNVVLOBNHIMQA-UHFFFAOYSA-N

Description

General description

Recombinant Buthus tamulus iberiotoxin expressed in E. coli. A single polypeptide chain of 37 amino acids that displays about 68% sequence homology with charybdotoxin (ChTx). Acts as a selective, reversible blocker of high conductance, Ca²⁺-activated K⁺ channels (PK,Ca). rIbTx does not block other types of voltage-dependent ion channels, especially other types of K⁺ channels that are sensitive to inhibition by ChTx. Shown to block PK,Ca in excised membrane patches from bovine aortic smooth muscle, acting exclusively at the outer face of the channel (IC₅₀ = 250 pM). Causes a decrease in probability of channel opening and the channel open mean time. rIbTx appears to modulate ChTx binding by an allosteric mechanism.

Recombinant Buthus tamulus iberiotoxin expressed in E. coli. Acts as a selective, reversible blocker of high conductance Ca²⁺-activated K⁺ channels (IC₅₀ = 250 pM).

Biochem/physiol Actions

Cell permeable: no

Primary Target
High conductance Ca2+-activated K+ channels

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: 250 pM against high conductance Ca2+-activated K+ channels

potently inhibits Ca²⁺-activated K⁺ channels expressed in Xenopus oocytes

Warning

Toxicity: Harmful (C)

Sequence

Pyr-Phe-Thr-Asp-Val-Asp-Cys⁷-Ser-Val-Ser-Lys-Glu-Cys¹³-Trp-Ser-Val-Cys¹⁷-Lys-Asp-Leu-Phe-Gly-Val-Asp-Arg-Gly-Lys-Cys²⁸-Met-Gly-Lys-Lys-Cys³³-Arg-Cys³⁵-Tyr-Gln-OH (disulfide bonds: 7 →28; 13 → 35; 17 → 33)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 3 months at -20°C or up to 2 weeks at 4°C.

Reconstitute in Tris-buffered saline (100 mM NaCl, 10 mM Tris-HCl, pH 7.5) containing 0.1% BSA.

Other Notes

Vanelli, G., et al. 1994. J. Appl. Physiol.76, 1098.
Garcia, M.L., et al. 1991. J. Bioeng. Biomembr.23, 615.
Galvez, A., et al. 1990. J. Biol. Chem. 265, 11083.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS06
• Signal Word: Danger
• Hazard Statements: H300 + H310 + H330
• Precautionary Statements: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3