382065

Bisbenzimide H 33342 Fluorochrome, Trihydrochloride

Cell-permeable, adenine-thymine-specific fluorescent stain.

• Synonym(s): Bisbenzimide H 33342 Fluorochrome, Trihydrochloride, 2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342
• Empirical Formula (Hill Notation): C₂₇H₂₈N₆O · 3HCl
• CAS Number: 23491-52-3
• Molecular Weight: 561.93
• UNSPSC Code: 12352111

Properties

• Quality Level: 100
• Assay: ≥95% (TLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: water: 50 mg/mL
• fluorescence: λ_ex 346 nm; λ_em 460 nm
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31)
• InChI key: PRDFBSVERLRRMY-UHFFFAOYSA-N

Description

General description

Cell-permeable adenine-thymidine specific fluorescent stain. Useful for staining DNA, chromosomes or nuclei for fluorescence microscopy and flow cytometry applications. DNA-bound dye has an excitation maximum of 358 nm and an emission maximum of 460 nm. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H33342 and the related compound H33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10 fold greater cell-permeability than H 33258.

Cell-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei for fluorescence microscopy and flow cytometry applications. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H 33342 and the related compound H 33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10-fold greater cell-permeability than H 33258.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Adenine-thymine-specific fluorescent stain

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Toxic & Carcinogenic / Teratogenic (G)

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions (1 mg/ml) are stable for up to 2 weeks at 4°C. Precipitates in phosphate buffer solutions.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Werstuck, G., and Green, M.R. 1998. Science 282, 296.
Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
Belloc, F., et al. 1994. Cytometry17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
Hinkley, R.E., et al. 1986. Dev. Biol.118, 148.
Sandhu, L.C., et al. 1985. Cytometry6, 191.
Frankfurt, O.S. 1983. Cytometry4, 216.
Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA78, 363.
Visser, J.W.M. 1980. Flow CytometryIV, 86.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable