317210

DM-NITROPHEN Reagent, Tetrasodium Salt

Caged Ca2+ chelator that undergoes a major and rapid decrease in Ca2+-binding affinity upon photolysis.

• Synonym(s): DM-NITROPHEN Reagent, Tetrasodium Salt, 1-(2-Nitro-4,5-dimethoxyphenyl)-1,2-diaminoethane-N,N,Nʹ,Nʹ-tetraacetic Acid, 4Na, Dimethoxynitrophenamine, 4Na
• Empirical Formula (Hill Notation): C₁₈H₁₉N₃O₁₂ · 4Na
• Molecular Weight: 561.32
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥93% (HPLC)
• form: solid
• reaction suitability: reagent type: chelator
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: orange
• solubility: water: 100 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Caged Ca²⁺ chelator that undergoes a major and rapid decrease in Ca²⁺-binding affinity upon photolysis. Can be microinjected or permeabilized into cells. May also be useful as a caged Mg²⁺ chelator. K_d (Ca²⁺) = 5 nM; K_d (Mg²⁺) = 2-5 µM.

Caged Ca²⁺ chelator that undergoes a major and rapid decrease in Ca²⁺-binding affinity upon photolysis. Can be microinjected or used with permeabilized cells. May also be useful as caged Mg²⁺ chelator. K_d (Ca²⁺) = 5 nM; K_d (Mg²⁺) = 2-5 µM.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Mulkey, R.M., and Zucker, R.S. 1993. J. Physiol. 462, 243.
Zucker, R.S. 1993. Cell Calcium 14, 87.
McCray, J.A., et al. 1992. Biochemistry31, 8856.
Delaney, K.R., and Zucker, R.S. 1990. J. Physiol.426, 473.
Kaplan, J.H., and Ellis-Davies, G.C. 1988. Proc. Natl. Acad. Sci. USA85, 6571.
Morad, M., et al. 1988. Science241, 842.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable