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17-1010

Sigma-Aldrich

Magna ChIP®-Seq ChIP and NGS Library Preparation Kit

chromatin IP & next generation sequencing library preparation from genomic DNA.

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About This Item

Quality Level

100

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
sample preparation: suitable

General description

ChIP-Seq facilitates genome-wide profiling of the interactions of transcription factors, modified histones, and other chromatin associated proteins. By combining chromatin immunoprecipitation with next generation sequencing, laboratories can generate high resolution data on protein-DNA interactions across the genome. For laboratories interested in gaining a deeper understanding of epigenetic mechanisms of gene regulation, ChIP-Seq has become an indispensable tool. The Magna ChIP-Seq Kit simplifies this technique by providing a validated set of reagents, easy-to-follow protocols, controls, and in process quality control guidelines to allow virtually any laboratory to conduct genome-wide analysis of DNA-protein interactions and the profiling of epigenetic marks.

Application

Magna ChIP-Seq Chromatin IP & Next Generation Sequencing Library Preparation Kit containing all key reagents for chromatin IP & next generation sequencing library preparation from genomic DNA.

Features and Benefits

  • Effective ChIP protocol simplifies production and immunoprecipitation of high quality chromatin
  • Reliable ChIP-Seq library construction from as little as 1 ng of purified ChIP DNA
  • Easy-to-follow protocols and in-process quality control guidelines help ensure successful ChIP and library construction in as little as 2 days.
  • Magna ChIP A+G bead blend allows the use of antibodies from virtually any species or class.
  • Enzymes and buffers provided in convenient master mix formulations streamlines library construction
  • Validate the ChIP procedure using inlcuded positive and negative control antibodies and a control primer set.
  • Proven performance through construction and sequencing of genomic DNA libraries on an Illumina Genome Analyzer II

Packaging

  • 10 library constructions.
  • Three modules containing all key reagents for chromatin immunoprecipitation and next generation sequencing library preparation from genomic DNA.

Components

  • Magna ChIP A/G (2° to 8°C) -Chromatin IP Reagents
  • Magna ChIP A/G (-20°C) -Chromatin IP Reagents
  • Magna ChIP-Seq Library Construction (-20°C) Reagents

Storage and Stability

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when used and stored as directed.

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

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