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Merck
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Key Documents

1.46714

Millipore

Tryptic Soy Broth Ready-to-use Bottles

Tween® 80 0.5 %, bottle capacity 125 mL, bottle filling volume 100 mL, closure type, blue flip cap and septum, sterile; autoclaved, suitable for sterility testing

Synonym(s):

CASO, Casein Soybean Digest Broth with 0.5 % Polysorbate, SCDB, Tryptic Soy Broth with 0.5 % Tween® 80

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About This Item

Agency

EP 2.6.1
JP 4.06
USP 71

Quality Level

300

sterility

sterile; autoclaved

form

liquid

feature

closure type blue flip cap and septum
ready-to-use

composition

Tween® 80, 0.5%

bottle capacity

125 mL

bottle filling volume

100 mL

pH

7.3±0.2

application(s)

cosmetics
food and beverages
pharmaceutical
sterility testing

storage temp.

2-25°C

suitability

nonselective for

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General description

Tryptic Soy Broth with 0.5% Tween® 80 is a nutrient rich inhibitor free microbial media designed in compliance with European (EP), Japanese (JP) and United States Pharmacopeia (USP).
The medium is supplemented with peptones from casein and soymeal to provide an enhanced carbon source, which promotes the growth of the microbes present in the test sample.
The neutralizing additives, Polysorbate (Tween®) 80reverses the growth inhibitory effect of most disinfectants and antiseptics. Neutralizing efficiency of histidine is effective against formaldehyde and formaldehyde releasing agents.
Additionally, Polysorbate 80 inactivates benzyl alcohol, carbanilides, dichlorbenzyl alcohols, benzoic acid, p-hydroxybenzoic acid and its esters, phenols, phenylethyl alcohols and Solbrols (PHB esters).

Application

Tryptic Soy Broth with 0.5% Tween® 80 is used for the isolation and cultivation of fastidious bacteria, yeast. TSB is suitable for sterility testing of substances, preparations, and products from pharmaceutical and food industry.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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