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Key Documents

05-351

Sigma-Aldrich

Anti-Fas Antibody

UPSTATE®, rat monoclonal, 7C10

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

product name

Anti-Fas Antibody, clone 7C10, clone 7C10, Upstate®, from rat

biological source

rat

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

7C10, monoclonal

species reactivity

mouse, bovine

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_152877

UniProt accession no.

P25445

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

bovine ... Fas(282488)

Specificity

Fas

Immunogen

recombinant protein containing the cytoplasmic domain of mouse Fas

Application

Anti-Fas Antibody, clone 7C10 is an antibody against Fas for use in IP & WB.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

routinely evaluated on RIPA lysates of mouse 3T3/A31 fibroblasts or membrane preparations of bovine brain

Target description

45kDa

Physical form

0.1 M Tris-glycine, 0.15 M NaCl, pH 7.4 with 0.05% sodium azide
Format: Purified
Protein G Chromatography

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Progesterone is a suppressor of apoptosis in bovine luteal cells.
Okuda, K; Korzekwa, A; Shibaya, M; Murakami, S; Nishimura, R; Tsubouchi et al.
Biology of reproduction null
Fas-mediated apoptosis is suppressed by calf serum in cultured bovine luteal cells.
Dariusz J Skarzynski, Masami Shibaya, Yukari Tasaki, Anna Korzekwa, Shuko Murakami et al.
Reproductive Biology null
Regulation of luteal function and corpus luteum regression in cows: hormonal control, immune mechanisms and intercellular communication.
D J Skarzynski,G Ferreira-Dias,K Okuda
Reproduction in domestic animals = Zuchthygiene null
Hua Wang et al.
Molecular medicine reports, 13(3), 2892-2898 (2016-01-29)
Hyperoxia is a high risk factor for neurodevelopmental disorders and can cause nerve cell death. 17β‑Estradiol (E2) has been demonstrated as a neuroprotective agent. In the present study, the effect of hyperoxia on rat oligodendrocyte precursor cells (OPCs) in vivo
Haiwei Mou et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(14), 3648-3653 (2017-03-23)
Genetic lesions that activate KRAS account for ∼30% of the 1.6 million annual cases of lung cancer. Despite clinical need, KRAS is still undruggable using traditional small-molecule drugs/inhibitors. When oncogenic Kras is suppressed by RNA interference, tumors initially regress but
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