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Key Documents

05-1417

Sigma-Aldrich

Anti-PTP-PEST Antibody, clone AG25

ascites fluid, clone AG25, from mouse

Synonym(s):

Protein-tyrosine phosphatase G1, Tyrosine-protein phosphatase non-receptor type12, Protein tyrosine phosphatase, non-receptor type 12

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

AG25, monoclonal

species reactivity

human, mouse, rat

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_002835

UniProt accession no.

Q05209

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PTPN12(5782)

General description

The protein tyrosine phosphatase PTP-PEST is an cytosolic protein tyrosine phosphatase that is ubiquitously expressed in mammalian tissues. PTP-PEST associates with multiple cytoskeleton and cell adhesion molecules suggesting a regulatory role in controlling cell shape and motility.

Specificity

Recognizes human PTP-PEST.

Immunogen

Baculovirus expressed purified human PTP-PEST

Application

Anti-PTP-PEST Antibody, clone AG25 detects level of PTP-PEST & has been published & validated for use in WB, IP & IC.
Immunoprecipitation: Recommended.

Immunocytochemistry: Recommended.

Quality

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PTP-PEST on 10 μg of HeLa lysates.

Target description

~88 kDa

Linkage

Replaces: MAB3739

Physical form

Mouse monoclonal IgG1 ascites containing no preservatives.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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