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Merck

10567752001

Roche

アルカリホスファターゼ

EIA grade, from calf intestine

別名:

AP, Alkaline Phosphatase

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About This Item

Enzyme Commission number:
UNSPSCコード:
12352204

由来生物

bovine (calf) intestine

品質水準

形状

solution

比活性

~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer)

包装

pkg of 1.5 mL (15 mg)

メーカー/製品名

Roche

最適pH

9.8

pH範囲

9.0-11.0

輸送温度

wet ice

詳細

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Amino groups: 8-16 moles/mole enzyme

アプリケーション

Alkaline phosphatase can be coupled to other proteins via its amino groups. The enzyme solution can be used directly for conjugation without prior dialysis.

品質

Purity: single peak (HPLC)

シーケンス

Purification procedure
AP from intestine is a dimeric glycoprotein, it is not known, whether the carbohydrate part is separated during purification procedure. Content of amino groupsIn the literature the carbohydrate content is 2 to 20 percent so one should find approx. 70 amino groups/mol.
In addition one researcher determined the amino groups and found 50 to 60 lysins/mol.-SH groups in AP
No information in literature about -SH groups in AP.

Carbohydrate structure
Results of investigation of the carbohydrate structure of several lots of alkaline phosphatase are available.

NH2- and carbohydrate estimation
Results of NH2- and carbohydrate estimation are available.

単位の定義

Unit Conversion: Approx. 4.35 U (EIA grade AP) [37 °C, 4-NPP as substrate, diethanolamine as buffer, pH 9.8] = 1 U [+25 °C, 4-NPP as substrate, glycine as buffer, pH 10.5]
1.0 U [+37 °C, QC assay conditions] = 0.73 U [+30 °C, QC assay conditions] = 0.48 U [+25 °C, QC assay conditions]

物理的形状

Solution, ready-to-use, 10mg/ml

調製ノート

Storage conditions (working solution): The diluted enzyme (1:10 in 3 M NaCl) will give a stability of 6 months at 4 °C.
1:10 dilution in water will give stability for 1 week at 4°C.
Do not freeze the diluted or undiluted enzyme, because freezing will destroy enzyme activity.

保管および安定性

Store at 2 to 8 °C. (Do not freeze)

その他情報

For life science research only. Not for use in diagnostic procedures.

WGK

WGK 1

引火点(°F)

does not flash

引火点(℃)

does not flash


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