06-942-AF555

Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor^™ 555 Conjugate

from rabbit, ALEXA FLUOR^™ 555

• 別名: H3K9ac, Histone H3, Lys9-acetylated, H3 histone family, member T, Lys9-acetylated, Lys9-acetylated histone 3, histone cluster 3, Lys9-acetylated, Lys9-acetylated H3
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: ALEXA FLUOR^™ 555
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: rat, mouse, human
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable
• NCBIアクセッション番号: NP_003520
• UniProtアクセッション番号: P68431
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: acetylation (Lys9)
• 遺伝子情報: human ... HIST1H3F(8968)

詳細

詳細

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

免疫原

KLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of histone H3 with acetylated Lys9.

アプリケーション

Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor^™ 555 Conjugate, Cat. No. 06-942-AF555, is a highly specific rabbit polyclonal antibody that targets histone H3 Lys9 acetylation (H3K9ac) and has been tested in Flow Cytometry and Immunocytochemistry.

Flow Cytometry Analysis: 2 µL from a representative lot detected cell population with histone H3 Lys9 acetylation (H3K9ac) among one million HEK293 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected nuclear histone H3 Lys9 acetylation (H3K9ac) in A431, HeLa, and NIH/3T3 cells.

The unconjugated antibody (Cat. No. 06-492) is shown to be suitable also for Western blotting, dot blot, flow cytometry, chromatin immunoprecipitation (ChIP) and ChIP-seq applications.

品質

Evaluated by Immunocytochemistry in HUVECs.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys9 acetylation (H3K9ac) in HUVECs.

ターゲットの説明

~17 kDa observed.

物理的形状

Purified rabbit polyclonal antibody in PBS with 1.5% BSA with 0.05% sodium azide.

その他情報

Concentration: Please refer to lot specific datasheet.

法的情報

ALEXA FLUOR is a trademark of Life Technologies

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable