HomeELISAcAMP Assays

cAMP Assays


We have prepared product lists for commonly used protocols from Cold Spring Harbor Protocols.

View the entire protocol on

Selected Protocol from "Protein-Protein Interactions", 2nd Edition, E. Golemis, P. Adam.

cAMP Assays

Gouzel Karimova and Daniel Ladant
Unite Postulante de Biochimie des Interactions Macromoleculaires, Departement de Biologie Structurale et Chimie,
CNRS URA 2185, Institut Pasteur, Paris, France


Buy this book, Z719145


cAMP measurements are obtained using an ELISA assay (Harlow and Lane 1988). Commercial radio-immunoassays, or ELISA kits, to assay cAMP can be purchased from various manufacturers. In our laboratory we use the homemade, less-expensive ELISA that is described below. In outline, this assay is based on the ability of soluble cAMP in bacterial extracts to compete with binding of an alkaline phosphatase (AP)-conjugated anti-cAMP antibody to immobilized cAMP. The surface-bound, immobilized anti-cAMP-AP is inversely correlated to the concentration of cAMP in the extract. Known concentrations of soluble cAMP are used to calibrate the assay.

View all available product lists for Cold Spring Harbor Laboratory Protocols

Product Association Disclaimer: The products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. Our products/reagents have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.