Cu C-N (Buchwald-Hartwig) cross-coupling kit was designed to provide the best possible chance of success and is run with:
Multiple tools have been created to ensure your success with kit set up. Start with the more detailed guide to ensure you are comfortable with all of the steps before using the quick guides on the excel worksheet. Remember that while the technique is new, it is still organic chemistry and so the steps will seem easy once you try just one kit. It is just a new way of approaching something you are already very good at.
Detailed Set-Up User Guide:
Designed for the first-time user and should be read completely before getting started. Best if used in conjunction with the video as not all steps are outlined in the video in great detail. This guide includes trouble-shooting tips, how-to’s for the Labware, and work-up recipes with procedures. Everything you need to set up a kit with confidence every time.
Each sheet is designed to be used with the specific experimental design chosen by you depending upon the attributes of your substrates. The downloadable excel files are specific to the kit being run and can be found within each Step-by-Step User Guide. They have the following features:
Contents in each of the 2 individually sealed Mylar (foil bags):
The screening sets come pre-loaded with 4 µmol of ligand in each vial according to the following design.
Figure 1.KitAlysis™ C-N Well Plate
Additional (not included) items needed:
Quench Solution Recipe
Note: This recipe makes 50 mL which is enough stock solution for all four screening sets in the KitAlysis™ Suzuki-Miyaura Cross-Coupling Reaction Screening Kit. The amount of internal standard is 10 mol%. So a big product peak to small internal standard indicates a good reaction.
Work-up Procedure and Analysis
Sealing the Plate-screwing down the cover
Sealing the plate properly is critical to success. The key is light, even pressure to the lid of the block to keep the cover flat while sealing.
Probe for hot plate does not fit into reaction block hole
Use a small oil bath or other metal block (for best results) in the back of the hot plate and place the probe in there. Place reaction block as close to the center of the hot plate as possible for more even stirring.
Due to narrow opening of pipette tips, slurry additions will result in blockages. To aid in uniform dosing, simply snip off the end of the tip at the first marker (~10 mm). To ensure an evenly dispersed aliquot, it is critical that the mixture is stirring well while you draw aliquots for dosing into the corresponding reaction vial.