HomeReaction Design & OptimizationStep-by-Step Guide for KitAlysis™ High-Throughput Metal Scavenger Screening Kit

Step-by-Step Guide for KitAlysis™ High-Throughput Metal Scavenger Screening Kit

2 x 12 pre-weighed scavengers / resins

Useful for:

Broad-spectrum metal removal

Impurity removal

Color treatment

Materials required – set up:

  • 1 mylar bag from the KitAlysis Metal Scavenger Reaction Screening Kit and you will use the following components
    • 2 x 12 pre-weighed scavengers / resins in glass vials topped with cap mat

Additional (not included) items needed:

  • 1 (1 mL) syringe with needle or 1 (1 mL) pipette with tips
  • 50 mL flask
  • Solvent (DCM, THF, EtOAc, DMF, MeOH, Toluene, Water (pH 2-10) or any other solvent in which your substrate is soluble)
  • Orbital shaker for 96 well plates (the use of stir bar is not recommended with some of the resins)
  • Your substrate: metal contaminated crude or purified material

Loading options:

Each kit can be used to screen 12 different scavengers, each at high (50 mg/vial) and low (10 mg/vial) loadings. Stock solution guidance for various w/w% is provided below. For instance, in a single kit, both 100 w/w% and 20 w/w% scavenger loading can be screened with a given stock solution.

Kit Components and Product Information

  • The screening sets come pre-loaded with 12 scavengers / resins (10mg or 50mg) in each vial according to the following design.
  • Individual kit components are available in research scale at


All contents in the foil bag are weighed, plated, packed, and sealed in a glove box under nitrogen.

Set Up Procedure:

  • Remove the silicon cap-mat from the plate and open each reaction vial carefully without changing its position on the plate. No pre-wetting or pre-activation of the resins is required.
  • Using a pipette or a syringe, add 1 mL of the stock solution to each reaction vial.
  • Re-cap all reaction vials.
  • Set the plate on the orbital shaker and stir for 4-16 hours (make sure you have adequate agitation). If you don’t have access to an orbital shaker, you can add stir bars to each vials and use a stir plate (this is not the preferred agitation method).
  • At the end of the stirring time, the plate can be centrifuged or the samples can be filtered using an inline syringe filter.
  • The metal scavenging process can be quantified by standard analytical techniques (ICP-OES, ICP-MS) by sampling the supernatant with a clean pipette.

Optimization tips:

  • Once the best scavenger and loading has been identified using the above procedure, a number of steps can be undertaken to further optimize the scavenging results.
    • Increasing reaction time
    • Increasing / decrease loading of the scavenger
    • Running the reaction at higher temperature
    • Screening different solvents
  • These steps can be done in standard glassware using bulk scavenger available from

Additional Recommended Materials (Sold Separately)

  • Z683809: 10-100 uL pipette
  • Z640220: 2-200 uL pipette tip refill
  • Z683825: 100-1000 uL pipette
  • Z640247: 50-1000 uL pipette tip refill
  • Z192570: 2 inch needles for ease in ampule solvent extraction
  • Z230723: 1 mL needle for accurate solvent volume extraction from ampule
  • 270997: Dichloromethane (anhydrous)
  • 270989: Ethyl acetate (anhydrous)
  • 186562: Tetrahydrofuran (anhydrous)
  • 227056: N,N-Dimethylformamide (anhydrous)
  • 244511: Toluene (anhydrous)