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  • Antibacterial, antioxidant and hypoglycaemic effects of Thymus capitatus (L.) Hoffmanns. et Link leaves' fractions.

Antibacterial, antioxidant and hypoglycaemic effects of Thymus capitatus (L.) Hoffmanns. et Link leaves' fractions.

Journal of enzyme inhibition and medicinal chemistry (2014-07-18)
Liliana Iauk, Rosaria Acquaviva, Silvana Mastrojeni, Andrea Amodeo, Michela Pugliese, Monica Ragusa, Monica R Loizzo, Francesco Menichini, Rosa Tundis
ABSTRACT

The aim of this study was to examine the bioactivity of the methanol fraction (MF) and n-hexane fraction (HF) of Thymus capitatus leaves in relation to their constituents analysed by gas chromatography and gas chromatography-mass spectrometry. The effects of T. capitatus on the growth of pathogenic bacteria associated with respiratory diseases (13 gram-positive and 4 gram-negative) were determined using a microdilution method. The MF was particularly effective on Streptococcus pneumoniae and Moraxella catarrhalis. The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), ferric-reducing antioxidant power and β-carotene bleaching assays. A strong activity using β-carotene bleaching test was observed with the MF (IC50 of 0.7 μg/mL after 30 min of incubation). In the hypoglycaemic test, a selective α-amylase inhibitory activity was detected with the HF begging the most active (IC50 of 422.5 μg/mL). T. capitatus may represent a source of natural bioactive compounds.

MATERIALS
Product Number
Brand
Product Description

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Methanol, HPLC Plus, ≥99.9%, poly-coated bottles
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Sodium sulfate, SAJ first grade, ≥99.0%, beads
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Sodium acetate, JIS special grade, ≥98.5%
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Ethanol, 94.8-95.8%
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Methanol, JIS 300, ≥99.8%, for residue analysis
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L-Ascorbic acid, JIS special grade, ≥99.0%
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Methanol, JIS special grade, ≥99.8%
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Sodium sulfate, for residue analysis
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Sodium acetate, SAJ first grade, ≥98.0%
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Methanol, SAJ first grade, ≥99.5%
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