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Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Advanced healthcare materials (2015-06-20)

Timothy M O'Shea, Matthew J Webber, Alex A Aimetti, Robert Langer

ABSTRACT

Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong and -ordered hydrogen bonding interactions within covalently incorporated trehalose hydrogels represent a possible mechanism for protein stabilization.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Ethyl acetate, ReagentPlus^®, ≥99.8%

Sigma-Aldrich

Ethyl acetate, ACS reagent, ≥99.5%

Sigma-Aldrich

Calcium chloride solution, BioUltra, for molecular biology, ~1 M in H₂O

Sigma-Aldrich

Dimethyl sulfoxide, anhydrous, ≥99.9%

Sigma-Aldrich

Ethyl acetate, anhydrous, 99.8%

Sigma-Aldrich

Ethyl thioglycolate, 97%

Sigma-Aldrich

Ethyl acetate, ≥99%, FCC, FG

Sigma-Aldrich

Calcium chloride, AnhydroBeads^™, −10 mesh, ≥99.99% trace metals basis

Sigma-Aldrich

Calcium chloride, anhydrous, powder, 99.99% trace metals basis

Sigma-Aldrich

Ethyl acetate, natural, ≥99%, FCC, FG

Sigma-Aldrich

Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer

Sigma-Aldrich

Aluminum oxide, nanowires, diam. × L 2-6 nm × 200-400 nm

Sigma-Aldrich

Calcium chloride, AnhydroBeads^™, −10 mesh, ≥99.9% trace metals basis

Sigma-Aldrich

Aluminum oxide, nanopowder, <50 nm particle size (TEM)

Sigma-Aldrich

Methanol-¹²C, 99.95 atom % ¹²C

Sigma-Aldrich

Aluminum oxide, 99.997% trace metals basis

Sigma-Aldrich

Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

Sigma-Aldrich

Dichloromethane, JIS 300, for residue analysis

Sigma-Aldrich

Dichloromethane, JIS special grade, ≥99.0%

Sigma-Aldrich

Calcium chloride, ≥75%

Sigma-Aldrich

Dichloromethane, SAJ first grade, ≥99.0%

Sigma-Aldrich

Ethyl acetate, JIS special grade, ≥99.5%

Sigma-Aldrich

Dimethyl sulfoxide, ≥99.0%, suitable for absorption spectrum analysis

Sigma-Aldrich

Ethyl acetate, suitable for HPLC

Sigma-Aldrich

Ethyl acetate, SAJ first grade, ≥99.0%

Sigma-Aldrich

Dimethyl sulfoxide, ≥99.5%

Sigma-Aldrich

Ethyl acetate, JIS 300, ≥99.5%, for residue analysis

Sigma-Aldrich

Calcium chloride, JIS special grade, ≥95.0%

Sigma-Aldrich

Dimethyl sulfoxide, suitable for HPLC

Sigma-Aldrich

Calcium chloride solution, 0.025 M