Skip to Content
Merck
  • Preventive effect of phytic acid on lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats.

Preventive effect of phytic acid on lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats.

Toxicology mechanisms and methods (2015-01-07)
E Brindha, M Rajasekapandiyan
PMID25560919
ABSTRACT

This study was aimed to evaluate the preventive role of phytic acid on lysosomal enzymes in isoproterenol (ISO)-induced myocardial infarction (MI) in male Wistar rats. Rats subcutaneously injected with ISO (85 mg/kg) at an interval of 24 h for two days showed a significant increase in the activities of lysosomal enzymes (glucuronidase, N-acetyl glucosaminidase, galactosidase, cathepsin-B and cathepsin-D) were increased significantly in serum and the heart of ISO-induced rats, but the activities of glucuronidase and cathepsin-D were decreased significantly in the lysosomal fraction of the heart. Pretreatment with phytic acid (25 and 50 mg/kg) daily for a period of 56 d positively altered activities of lysosomal hydrolases in ISO-induced rats. Thus, phytic acid possesses a cardioprotective effect in ISO-induced MI in rats.

MATERIALS
Product Number
Brand
Product Description
M8787
Sigma-Aldrich
Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR
2104
Supelco
Hydrochloric acid solution, volumetric, 0.1 M HCl (0.1N), endotoxin free
L4509
Sigma-Aldrich
Sodium dodecyl sulfate, ReagentPlus®, ≥98.5% (GC)
L6026
Sigma-Aldrich
Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)
L5750
Sigma-Aldrich
Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis
13-1650
Sigma-Aldrich
Hydrochloric acid, SAJ first grade, 35.0-37.0%
28-2320
Sigma-Aldrich
Sodium chloride solution, 0.1 M
28-3284
Sigma-Aldrich
Sodium dodecyl sulfate, ≥99.0%
28-3260
Sigma-Aldrich
Sodium dodecyl sulfate, SAJ special grade, ≥97.0%
28-3285
Sigma-Aldrich
Sodium dodecyl sulfate, ≥99.0%
28-2280
Sigma-Aldrich
Sodium chloride, SAJ first grade, ≥99.0%
28-3270
Sigma-Aldrich
Sodium dodecyl sulfate, SAJ first grade, ≥95.0%
28-2300
Sigma-Aldrich
Sodium chloride solution, 1 M
13-1770
Sigma-Aldrich
Hydrochloric acid solution, SAJ first grade, 9.5-10.0%
13-1630
Sigma-Aldrich
Hydrochloric acid, SAJ super special grade, ≥35.0%
28-2270
Sigma-Aldrich
Sodium chloride, JIS special grade, ≥99.5%
13-1640
Sigma-Aldrich
Hydrochloric acid, JIS special grade, 35.0-37.0%
705551
Sigma-Aldrich
Hydrogen chloride solution, 3 M in cyclopentyl methyl ether (CPME)
75746
Sigma-Aldrich
Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets
00457
Sigma-Aldrich
Magnesium chloride solution, 0.1 M
71729
Sigma-Aldrich
Sodium dodecyl sulfate, ≥98.0% (GC)
84429
Sigma-Aldrich
Hydrochloric acid solution, ~6 M in H2O, for amino acid analysis
55422
Sigma-Aldrich
Sodium dodecyl sulfate, ≥98.0% (GC)
71726
Supelco
Sodium dodecyl sulfate, suitable for ion pair chromatography, LiChropur™, ≥99.0%
440469
Sigma-Aldrich
Lithium lactate, 95%
71717
Sigma-Aldrich
Sodium dodecyl sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate
71387
Supelco
Sodium chloride, reference material for titrimetry, certified by BAM, >99.5%
62862
Sigma-Aldrich
Sodium dodecyl sulfate, ≥90% ((Assay))
451940
Sigma-Aldrich
Sodium chloride-35Cl, 99 atom % 35Cl
378860
Sigma-Aldrich
Sodium chloride, random crystals, optical grade, 99.9% trace metals basis