The polymerase chain reaction (PCR) is a powerful core molecular biology technique. It is an efficient and rapid in vitro method for enzymatic amplification of specific DNA or RNA sequences from nucleic acids of various sources. A simple PCR reaction consists of a set of synthetic oligonucleotide primers that flank the target DNA sequence, target DNA, a thermostable DNA polymerase and dNTPs. A repetitive series of cycles involving template denaturation, primer annealing, followed by extension of the annealed primers, yields tremendous amounts of DNA. Because the strands synthesized in one cycle serve as a template in the next, a million-fold increase in the DNA amount is achieved in just 20 cycles. From PCR microplates, plate covers, pipette tips, PCR tubes, and thermocyclers, explore our comprehensive offering of PCR consumables and equipment to support every step of your PCR workflow.
A thermal cycler (also known as a PCR machine or thermocycler) is a laboratory instrument that heats and cools samples in repetitive cycles to facilitate DNA or RNA amplification through the polymerase chain reaction. These machines have become commonplace in labs, although the introduction of mini or portable thermal cyclers has made it easier to perform PCR reactions in the field and classroom. Many thermal cyclers are programmable and allow users to save frequently used protocols. PCR typically requires 20 to 35 cycles comprising two to three temperature steps (denaturation, annealing, and extension). Combining speed, flexibility, and PCR optimization, the Eppendorf® Mastercycler Thermocyclers are suitable for enhanced PCR functions, like the 2D-Gradient and thorough documentation capabilities. Additionally, we also offer Benchmark Scientific TC models of thermocyclers that are designed for lower throughput needs, while maintaining the same functionality and a lower price point compared to larger units.
Additionally, discover a variety of additional PCR plate seals, including eXTReme™ plate seals, ThermalSeal RTS™ plate seals, and AlumaSeal® PCR plate seals to accommodate your PCR workflow needs.
With BRAND® PCR products you can protect your samples from contamination and evaporation. Cleanroom production, high-quality raw materials, and seamless quality controls by accredited laboratories ensure consistently high standards for cleanliness. Sealing films and covers made to fit plates and tubes significantly reduce evaporation loss and allow for reliable analyses, even with small sample volumes. BRAND® PCR consumable materials are always free from DNase, DNA, RNase, and endotoxins. We also offer Eppendorf® twin-tec PCR plates that combine the advantages of using both polypropylene and polycarbonate materials while the Corning® Thermowell PCR 96 well plates are suitable for the majority of thermocylers and ideal for high-throughput automation.
Explore a variety of reliable pipet tips, including market-leaders such as ART® pipet tips in our product selection table. In addition, BRAND® pipette tips and filter tips are manufactured in a cleanroom under the most modern production conditions and are automatically rack packed and packaged to ensure that the tips are of consistently high quality. BRAND® pipette tips and filter tips are manufactured with high-purity polypropylene, free from DiHEMDA, oleamide, DNA (< 40 fg), RNase (< 8.6 fg), endotoxins (< 1 pg) and ATP (< 1 fg). Additionally, we are proud to offer a portfolio of Eppendorf® products such as Eppendorf pipette tips that are used for liquid handling applications. They are useful for speed processing and avoiding cross-contamination. We offer a broad range of Eppendorf® pipette tips for niche applications according to the volume capacities, sterile and non-sterile demands as well as racking. Furthermore, Axygen consumables include not only pipette tips but also a variety of microplate options to match your specific application needs.