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Cytiva™

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Cell separation media such as Ficoll-Paque® and Percoll® medias.

Cell Separation Media

Cytiva™ products for bioprocess and life science research.
Cy3® and Cy5® fluorescent labels.
Illustra™ Nucleic Acid Preparation
Whatman Blotting Papers, Membranes, & FTA Cards
ECL Western Blotting Products

Cytiva™ technologies have enabled breakthroughs in science and medicine that have shaped today's biotechnology industry. With a broad portfolio that includes Whatman® filters and membranes, Amersham™ chromatography columns and resins, tissue culture reagents, and products for sample preparation, blotting, and protein analysis, Cytiva™ advances the discovery and manufacture of therapeutics.   

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Membranes
Image of Kits
Kits
Image of Columns
Columns
Image of Plates
Plates
Image of Filters
Filters
Image of Centrifugal Filters
Centrifugal Filters

Whatman® lab filtration products

Whatman® products are used for laboratory processes from basic sample filtration to high-throughput automated applications to demanding HPLC sample prep.

  • Filter paper, membrane filter, and syringe filter lab basics in materials, sizes, shapes and pore sizes
  • Automation-friendly Mini-UniPrep™ syringeless filters and vials, track-etched and Anopore™ membranes meet the stringent requirements of pharmaceutical companies.
  • Essential for environmental monitoring and air, water and soil testing, Whatman® products are cited in EPA, ASTM and ISO protocols.

Amersham™ resins and pre-packed columns for protein purification

Cytiva™ offers a variety of columns and resins for lab scale to process scale antibody and protein purification including solutions for affinity, ion exchange, and size exclusion chromatography.

  • GraviTrap™ columns are prepacked gravity-flow columns designed for fast and efficient manual purification of proteins and antibodies from cell culture supernatants and biological fluids.
  • SpinTrap™ columns are prepacked, single-use spin columns for simple, small-scale purification of proteins and antibodies.
  • HiTrap™ HP columns are prepacked with Sepharose™ High Performance resins for routine, preparative purification of proteins and antibodies.
  • MultiTrap™ 96-well filter plates provide a fast, reliable format for small-scale purification of antibodies or enrichment of proteins of interest using prepacked 96-well filter plates amenable to centrifugation or vacuum filtration, manually or using automated robotic systems.
  • HiScreen™ columns are part of the process development platform, and provide an excellent choice for method optimization and parameter screening.
  • Mag Sepharose™ media are comprised of sepharose-based magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications, and can be used for rapid, small-scale purification and screening of proteins and antibodies from serum and cell supernatants.
  • Capto™ HiRes™ prepacked columns for high-resolution purification of proteins and other biomolecules by ion exchange chromatography are well-suited for the preparation of highly pure proteins for structural studies and for protein analysis.
  • Sephacryl™, Superose™, and Sephadex™ prepacked gel filtration columns are available for size exclusion chromatography.
  • Sepharose™ and Sepharose™ Fast Flow resins are also available separately for scaling procedures.
    • Membranes, reagents, and equipment for Western blotting and chemiluminescent or fluorescent protein detection
    • Equipment and reagents for SDS-PAGE and isoelectric focusing (IEF)
    • Kits and buffers for protein lysis, extraction, and clean-up of proteins from tissues and cells

Protein sample preparation and analysis

Cytiva™ offers a comprehensive toolbox for analyzing proteins by electrophoresis, blotting, and imaging.

Cell analysis tools

Visualize, analyze, and realize your research goals with tools for high content analysis, high resolution microscopy, and super resolution imaging.

Cell therapy and cell processing reagents

High-quality media and reagents are key to consistent production. Cytiva™ products including density gradient media, cell culture media, and reagents yield robust and reproducible batches. This reliability minimizes the need for costly process changes or revalidations.

Related Product Resources

Whatman® Lab Filtration Product

Tech Article: RNA isolation from blood, cultured cells, and animal or plant tissue on Whatman® FTA™ cards
Protocol: Reliable extraction of DNA from Whatman® FTA™ cards

2-D Electrophoresis

Tech Article: Troubleshooting 2-D DIGE Results
Protocol: Processing Small Tissue or Cell Samples using Sample Grinding Kit
Protocol: Protease Inhibition using Protease Inhibitor Mix
Protocol: Two-Dimensional Separation of Protein Samples
Protocol: Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System
Protocol: Desalting Samples Using Mini Dialysis Kit
Protocol: Cleaning Up Samples using 2-D Clean-Up Kit
Protocol: Protein Determination using 2-D Quant Kit

Related Product Categories
Laboratory Sample Collection
Protect and manage your samples with solutions including vials, tubes, bottles, dishes, plates, boxes, and racks for collection, transport, tracing, and storage needs.
Syringe Filters
High performance sterile and nonsterile syringe filters offered in a variety of membrane materials and pore sizes, tailored for sample preparation for HPLC, UHPLC, ion chromatography, dissolution testing, and other analyses.
Filter Paper
Whatman® filter papers for qualitative and quantitative analysis.
Filter Membranes
Enhance HPLC and analysis with membranes filtering solvents, aqueous solutions. Ideal for prep, clarity, removal, sterilization, separation.
Recombinant/Fusion Tag Protein Purification
Optimize protein purification with our range of affinity resins and buffers for multiple tags and purification techniques.
Antibody Protein Purification
Antibody purification resins and prepacked columns for lab scale, process scale, and industry scale workflows.
Biotinylated Protein Purification
Streptavidin and avidin resins and prepacked columns for small- to large-scale purification of biotinylated proteins and antibodies.
Cell Culture Supplements & Reagents
Browse our selection of high-quality cell culture supplements and reagents, including sera, attachment factors, growth factors, cytokines, hormones, cell dissociation reagents, and cell freezing reagents.
Related Applications
Cell Therapy Manufacturing
Introducing a new cell therapy demands optimal performance in upstream and downstream processing, process development, testing, and manufacturing. World-class products and partners are critical to success, speeding advancement of therapeutics that are effective, safe, and affordable.
Monoclonal Antibody Manufacturing
Monoclonal antibody manufacturing is a highly templated approach used to produce mAb-based immunotherapies. Robust, scalable process solutions are required at every step to ensure high therapeutic concentration and process safety, while meeting speed-to-market and cost containment concerns.
Imaging Analysis & Live Cell Imaging
An overview of current tools and reagents for live cell imaging and analysis with applications in cell trafficking, cell health, gene expression analysis, cell migration, 3D cell culture, cytoskeletal dynamics and more.
Lysis & Protein Extraction
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Sample Preparation by Filtration
Filtration separates substances based on physical or chemical properties. Lab samples are routinely filtered before analysis to reduce sample complexity and increase analyte purity. This allows scientists to generate higher quality analytical results.
Protein Purification
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
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