581424-U

Ascentis^® C8 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

• Synonym(s): C8 Reversed-Phase Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Ascentis^® C8 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
• material: stainless steel column
• Agency: suitable for USP L7
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• extent of labeling: 15% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 15 cm × 4.6 mm
• surface area: 450 m²/g
• surface coverage: 4.0 μmol/m²
• impurities: <5 ppm metals
• matrix: fully porous particle; silica gel high purity, spherical
• matrix active group: C8 (octyl) phase
• particle size: 5 μm
• pore size: 100 Å
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

The Ascentis C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excells in highly aqueous mobile phases as well as high organic mobile phases.

Application

• Analysis of 27 beta-Blockers and Metabolites in Milk Powder by High Performance Liquid Chromatography Coupled to Quadrupole Orbitrap High-Resolution Mass Spectrometry.: This study utilizes the Ascentis^® C8 HPLC Column to analyze beta-blockers and their metabolites in milk powder, highlighting the column′s capacity for sensitive and precise quantification in complex matrices (Cheng et al., 2019).


• Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: A review of advancements in HPLC technology, including the use of Ascentis^® C8 columns, which enhance the speed and efficiency of chemical separations (Ali et al., 2012).


• Superficially porous particles columns for super fast HPLC separations.: Discusses the benefits of using Ascentis^® C8 HPLC Columns for achieving faster and more efficient separations in HPLC, underlining their utility in high-throughput settings (Ali et al., 2012).


• Gradient RP-HPLC method for the determination of potential impurities in atazanavir sulfate.: Demonstrates the effectiveness of the Ascentis^® C8 HPLC Column in a robust method for identifying impurities in pharmaceutical compounds, ensuring product safety and compliance (Chitturi et al., 2011).


• Development and validation of an HPLC-MS/MS method to determine clopidogrel in human plasma. Use of incurred samples to test back-conversion.: Illustrates the application of Ascentis^® C8 HPLC Column in a validated method for the analysis of clopidogrel, improving therapeutic drug monitoring and patient care (Silvestro et al., 2010).

Features and Benefits

• Superior retention for hydrophobic molecules
• Excellent peak shape
• Low bleed LC-MS separations

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable