565325-U

Ascentis^® RP-Amide (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

• Synonym(s): RP-Amide Reversed-Phase Chromatography Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Ascentis^® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L60
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• extent of labeling: 19.5% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 25 cm × 4.6 mm
• surface area: 450 m²/g
• surface coverage: 2.7 μmol/m²
• impurities: <5 ppm metals
• matrix: fully porous particle; silica gel high purity, spherical
• matrix active group: amide, alkyl phase
• particle size: 5 μm
• pore size: 100 Å
• operating pH range: 2-8
• application(s): environmental; food and beverages
• separation technique: reversed phase

Description

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Application

• A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: This study presents a validated Ultra-High-Performance Liquid Chromatography (UHPLC) method employing an RP-Amide fused-core column for the quantitative determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. The method demonstrated excellent precision, accuracy, and sensitivity, making it a valuable tool for the analysis of these bioactive compounds in complex matrices. The research highlights the utility of the Ascentis^® RP-Amide HPLC Column in enhancing the resolution and efficiency of chromatographic separations in analytical chemistry applications (Fibigr et al., 2018).

Features and Benefits

• Excellent retention and peak shape for polar compounds
• 100% aqueous compatibility
• Ultra low bleed, LC-MS compatible
• Unique selectivity

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable