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Supel QuE

Z-Sep+ Bulk, pkg of 20 g

EC Number:


polypropylene tube

Quality Level

product line



Z-Sep+ Bulk


pkg of 20 g


QuEChERS: suitable


silica gel base material

matrix active group

zirconia-based phase

particle size

50 μm

pore size

70 Å pore size

surface area

480 m2/g

Featured Industry

Food and Beverages

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General description

Dispersive SPE (dSPE), often referred to as the "QuEChERS" method (Quick, Easy, Cheap, Effective, Rugged, and Safe), is modern sample prep technique that is becoming increasingly popular in the area of multi-residue pesticide analysis in food and agricultural products.

Using the QuEChERS method, food/agricultural samples are first extracted with an aqueous miscible solvent (e.g., acetonitrile) in the presence of high amounts of salts (e.g., sodium chloride and magnesium sulfate) and/or buffering agents (e.g. citrate) to induce liquid phase separation and stabilize acid and base labile pesticides, respectively. Upon shaking and centrifugation, an aliquot of the organic phase is subjected to further cleanup using SPE. Unlike traditional methods using SPE tubes, in dispersive SPE, cleanup is facilitated by mixing bulk amounts of SPE (e.g., Supelclean PSA, ENVI-Carb, and/or Discovery DSC-18) with the extract. After sample cleanup, the mixture is centrifuged and the resulting supernatant can either be analyzed directly or can be subjected to minor further treatment before analysis.

Supelco carries a line of vials and centrifuge tubes containing pre-determined amounts of salts and SPE sorbents to support the most common method configurations used today.

Other Notes

SupelQuE Z-Sep+ enhances sample cleanup for complex matrices by effectively removing more fat and color from sample extracts than traditional phases for QuEChERS LC-MS methods. By eliminating problematic matrix interferences, Z-Sep products provide more robust LC-MS methods. This proprietary technology can replace C18 and PSA in your current methods without additional method development.

Z-Sep+ is recommended for cleanup of samples containing greater than 15% fat.

Legal Information

Certain uses of this product may be covered by the claims of one or more pending patent applications. For licensing inquiries please contact
Supel is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Lianzhu Wang et al.
Se pu = Chinese journal of chromatography, 33(5), 501-507 (2015-09-22)
A fast method based on QuEChERS methodology for the simultaneous determination of 32 sulfonylurea herbicide residues in sweet corns and green soybeans was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The clean-up effects of three dispersive sorbents were evaluated in...
Darina Lankova et al.
Analytical and bioanalytical chemistry, 408(10), 2515-2525 (2016-02-11)
In this study, a novel analytical approach for the determination of 11 monohydroxylated polycyclic aromatic hydrocarbon metabolites (OH-PAHs) in urine was developed and validated. The rapid, simple and high-throughput sample preparation procedure based on ethyl acetate extraction and subsequent purification...
Jonatan V Dias et al.
Journal of chromatography. A, 1462, 8-18 (2016-08-11)
The goal of this work was to evaluate the efficiency of several sorbents on removal fats from edible oils (olive, soya and sunflower) during the clean-up step for posterior determination of 165 pesticides by UHPLC-QqQ-MS/MS system. The extraction procedure employed...
Bruce D Morris et al.
Journal of agricultural and food chemistry, 63(21), 5107-5119 (2015-02-24)
A new, automated, high-throughput, mini-column solid-phase extraction (c-SPE) cleanup method for QuEChERS extracts was developed, using a robotic X-Y-Z instrument autosampler, for analysis of pesticide residues in fruits and vegetables by LC-MS/MS. Removal of avocado matrix and recoveries of 263...
Ana Lozano et al.
Talanta, 118, 68-83 (2013-11-28)
Two sorbents containing ZrO₂ (Z-Sep and Z-Sep+) were tested as a d-SPE clean-up in combination with the QuEChERS and ethyl acetate multiresidue method in the pesticide residues extraction in avocado. All extracts were analysed using gas chromatography coupled with a...


Increase Removal of Fat and Pigment from Avocado Extracts Prior to GC-MS Analysis of Pesticide and Metabolite Residues

Supelco's Z-Sep+ sorbent effectively removes fat and pigment interferences from avocado, with good pesticide recoveries; it removes more matrix than traditional PSA/C18 sorbent, and produces a lower background for GC-MS analysis.

GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Salmon on SPB®-608 after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis

GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Salmon on SPB®-608 (20 m x 0.18 mm I.D., 0.18 µm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis

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