ZRB2968

Anti-Homer 1 Antibody, clone 1F5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): SYN47
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1F5, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 40.28 kDa; observed mol wt ~51 kDa
• purified by: using Protein A
• species reactivity: human, rat, mouse
• species reactivity (predicted by homology): bovine, monkey, avian, canine, (Whale), horse
• packaging: antibody small pack of 25
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_004263.1
• UniProt accession no.: Q86YM7
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... HOMER1(9456)

Description

General description

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1F5 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Homer-1. It targets an epitope within 17 amino acids from the C-terminal region of human Homer-1.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of Homer-1.

Application

Quality Control Testing

Evaluated by Western Blotting in Neuro-2a cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Homer-1 in Neuro-2a cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Homer-1 in lysates from SH-SY5Y cells and Human brain tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Homer-1 in Rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Homer-1 in SH-SY5Y cells.

Affinity Binding Assay: A representative lot of this antibody bound Homer-1 peptide with a KD of 1.0 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Homer protein homolog 1 (UniProt: Q86YM7; also known as Homer-1) is encoded by the HOMER1 (also known as SYN47) gene (Gene ID: 9456) in human. Homer protein homolog 1 (Homer-1) is a scaffolding protein involved in intracellular signaling pathways and neuronal function. It plays a critical role in receptor clustering, cell signaling modulation, and synaptic plasticity. Homer-1 contains an N-terminal coiled-coil region that allows for the formation of homo- and heteromeric interactions with other proteins, while the C-terminal domain binds to various target proteins involved in synaptic signaling. Homer-1 interacts with several proteins such as metabotropic glutamate receptors (mGluRs), inositol trisphosphate receptors (IP3Rs), and Shank proteins. Homer-1 is widely expressed in various tissues, but it is highly enriched in the brain, particularly in regions associated with synaptic transmission, such as the cortex, hippocampus, and cerebellum. Dysregulation of Homer-1 expression and function has been implicated in various neurological disorders, including schizophrenia, Alzheimer′s disease, epilepsy, and drug addiction. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Luo, P., et al., (2012) Neurochem. Int. 61, 731 738)

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.