ZRB2841

Anti-YB1 Antibody, clone 1G7 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): CCAAT-binding transcription factor I subunit A (CBF-A), DBPB, DNA-binding protein B, EFI-A, Enhancer factor I subunit A, NSEP1, Nuclease-sensitive element-binding protein 1, Y-box transcription factor, Y-box-binding protein 1, YB1
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1G7, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 36 kDa; observed mol wt ~50 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgGκ
• epitope sequence: N-terminal
• Protein ID accession no.: NP_004550
• UniProt accession no.: P67809
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... YBX1(4904)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1G7 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects YB1. It targets an epitope within 23 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal of human YB1.

Application

Quality Control Testing

Evaluated by Western Blotting in T-47D cell lysate.

Western Blotting Analysis: A 1:25,000 dilution of this antibody detected YB1 in T-47D cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected YB1 in C6 and human testis lysates.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected YB1 in HEK-293 cells.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected YB1in HeLa cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected YB1 in Human testis tissue sections.

Flow Cytometry Analysis: 0.1 µg of this antibody detected YB1 in one million fixed and permeabilized HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Y-box-binding protein 1 (UniProt: P67809; also known as YB-1, CCAAT-binding transcription factor I subunit A (CBF-A), DNA-binding protein B, Enhancer factor I subunit A, EFI-A, Nuclease-sensitive element-binding protein 1, Y-box transcription factor) is encoded by the YBX1 (also known as NSEP1, YB1) gene (Gene ID: 4904) in human. Y-box-binding protein 1 (YB-1) belongs to the cold shock domain (CSD) protein family, which is characterized by a highly conserved nucleic acid-binding domain. YB-1′s structure includes the CSD at the N-terminus and a diverse C-terminal domain that contains both alternating basic and acidic regions. This structure facilitates its role in binding to both DNA and RNA, enabling a wide range of functions. YB-1 is a multifunctional protein that plays significant roles in various cellular processes including regulation of transcription, translation of mRNAs, DNA repair, and stress response. It acts as a transcription factor that can both activate and repress genes. In translation, YB-1 binds to mRNA and can affect its stability and translation efficiency, often in response to cellular stress. YB-1 is ubiquitously expressed across various tissues but shows higher expression levels in lung, colon, and breast tissues. Its overexpression has been observed in many cancers, including breast, ovarian, lung, and prostate cancers, and is often linked with aggressive tumor growth, increased metastasis, and resistance to treatment. Additionally, YB-1 has been implicated in other pathological conditions such as inflammation and fibrosis.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable