ZRB2709

Anti-LRP2 Antibody, clone 1I21 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Glycoprotein 330 (gp330), LRP-2, Megalin
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1I21, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 522 kDa; observed mol wt ~500 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal cytoplasmic domain
• Protein ID accession no.: NP_004516
• UniProt accession no.: P98164
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... LRP2(4036)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1I21 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Low-density lipoprotein receptor-related protein 2 (LRP2). It targets an epitope within 24 amino acids from the C-terminal cytoplasmic domain of the protein.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal cytoplasmic domain of human LRP2.

Application

Quality Control Testing

Evaluated by Immunohistochemistry in human kidney.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected LRP2 in human kidney sections.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected LRP2 in human kidney membrane lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LRP2 in Caco-2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Low-density lipoprotein receptor-related protein 2 (UniProt: P98164; also known as LRP-2, Glycoprotein 330 (gp330), Megalin) is encoded by the LRP2 gene (Gene ID: 4036) in human. LRP2 is a large transmembrane receptor protein that is involved in receptor-mediated endocytosis. It is primarily expressed in absorptive epithelial cells of various tissues, including the kidney, thyroid, and intestine. LRP2 plays a crucial role in the reabsorption of small proteins and protein-bound ligands from the glomerular filtrate in the kidney, as well as in the endocytosis of various molecules in other tissues. Additionally, it is expressed in the central nervous system and is implicated in developmental processes, including embryonic neural tube closure and eye development. Mutations in the LRP2 gene is associated with Donnai-Barrow syndrome, and various tumor development processes. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable