ZRB2329

Anti-KIFC1 Antibody, clone 3M19 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Kinesin-like protein 2, Kinesin-like protein KIFC1, Kinesin-related protein HSET
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: Clone, 3M19
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 73.75 kDa; observed mol wt ~74 kDa
• purified by: using Protein A
• species reactivity: human, mouse
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_002254
• UniProt accession no.: Q9BW19
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... KIFC1(3833)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3M19 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Kinesin-like protein KIFC1. It targets an epitope within 22 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal region of human Kinesin-like protein KIFC1.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected KIFC1 in HeLa cell nuclear extract.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected KIFC1 in NIH3T3 cell nuclear extract.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected KIFC1 in Human testis tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound KIFC1 peptide with a KD of 1.3 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected KIFC1 in U-251 MG cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Kinesin-like protein KIFC1 (UniProt: Q9BW19; also known as Kinesin-like protein 2, Kinesin-related protein HSET) is encoded by the KIFC1 (also known as HSET, KNSL2) gene (Gene ID: 3833) in human. KIFC1 is a minus end-directed motor protein, which is critical in centrosome clustering and is required for bipolar spindle formation. It is detected in the nucleus, cytoplasm, and early endosomes. It is shown to be associated with nucleus during interphase, centrosomes in early and spindle in later mitosis. Its conserved kinesin motor domain is localized in amino acids 310-663 and contains the microtubule-binding site and a catalytic region for the hydrolysis of ATP. Its tail domain can specifically recognize and bind to the membranous organelles, vesicles, and -tubulin. It is reported to transport various cargos along the microtubule to the minus ends. Deletion of KIFC1 gene leads to defects in metaphase mitotic spindle assembly, and results in chromosome structural abnormalities. Overexpression of KIFC1 is reported in patients with hepatocellular carcinoma and is linked to disease progression and poor prognosis. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wei, Y-L., and Yang, W-X. (2019). Cell Death Dis. 10; Article 402; Fu, X., et al. (2018). Int. J. Oncology. 52(6); 1912-1922).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable