ZRB1768

Anti-Claudin-1 Antibody, clone 1O12 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Senescence-associated epithelial membrane protein
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1O12, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 22.74 kDa; observed mol wt ~18 kDa
• purified by: using Protein A
• species reactivity: canine, human
• species reactivity (predicted by homology): monkey, feline, bovine
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_066924
• UniProt accession no.: O95832
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CLDN1(9076)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1O12 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Claudin-1. It targets an epitope within 24 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human Claudin-1.

Application

Quality Control Testing

Evaluated by Western Blotting in MDCK cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Claudin-1 in MDCK cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Claudin-1 in A431, A549 cell lysates and 1:10,000 dilution detected Claudin-1 in RT4 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound Claudin-1 with a KD of 1.0 x 10-12 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Claudin-1 in human skin tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Claudin-1 in A431 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Claudin-1 (UniProt: O95832; also known as Senescence-associated epithelial membrane protein) is encoded by the CLDN1 (also known as CLD1, SEMP1, UNQ481/PRO944) gene (Gene ID: 9076) in human. Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are co-expressed and interact with each other, and this determines the overall permeability. Claudin-1 is a multi-pass membrane protein with three cytoplasmic domains, four transmembrane domains, and two extracellular domains. It is strongly expressed in liver and kidney and its expression is also observed in heart, brain, spleen, lung, and testis. It can form homo- and heteropolymers with other claudin family members. It also directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 and residues 210 and 211 are required for this interaction. Claudin-1 is shown to be essential for preventing the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. It is required for normal water homeostasis and prevents excessive water loss through the skin. In human liver cells, claudin-1 is reported to promote invasive behavior by activating the c-Abl-PKC signaling pathway. CLDN1 knock-out mice die at the first day of birth due to increased trans-epidermal water loss and human subjects lacking claudin-1 suffer from the Neonatal Ichthyosis-Sclerosing Cholangitis (NISCH) syndrome that includes an ichthyosiform skin phenotype. Claudin-1 is also known to associate with CD81 and the claudin-1-CD81 receptor complex is reported to be essential for hepatitis C virus (HCV) entry into host cell. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bergmann, S., et al. (2020). Sci. Rep. 10; article 2024; Singh, AB., et al. (2017). Pflugers Arch. 469(1): 69-75; Harris, HJ., et al. (2010). J. Biol. Chem. 285(27); 21092-21102).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable