ZRB1754

Anti-TP63 Antibody, clone 1E14 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Tumor protein 63;p63;Chronic ulcerative stomatitis protein;CUSP;Keratinocyte transcription factor KET;Transformation-related protein 63;Tumor protein p73-like;p40;p73L;P51
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1E14, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 65.76 kDa; observed mol wt ~66 kDa
• purified by: using Protein A
• species reactivity: human, mouse
• species reactivity (predicted by homology): rat
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_001108452
• UniProt accession no.: Q9H3D4-2
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... TP63(8626)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1E14 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Tumor protein 63 (TP63). It targets an epitope within 13 amino acids from the N-terminal region. It is predicted to react with isoforms 2, 4, 8, and 12.

Immunogen

KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of isoform 2 of human Tumor protein 63 (TP63).

Application

Quality Control Testing

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TP63 in A431 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TP63 in Mouse E18 skin tissue lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TP63 in A431 cells.

Affinity Binding Assay: A representative lot of this antibody bound TP63 peptide with a KD of 6.9 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TP63 in A431 cell lysate.

Target description

Tumor protein 63 (UniProt: Q9H3D4; also known as p63, Chronic ulcerative stomatitis protein, CUSP, Keratinocyte transcription factor KET, Transformation-related protein 63, TP63, Tumor protein p73-like, p73L, P40, P51) is encoded by the TP63 (also known as KET, P63, P73H, P73L, TP73L) gene (Gene ID: 8626) in human. TP63 is a member of the p53 protein family that is widely expressed. It is structurally related to the p53, however its C-terminal region differs from p53′s in that it contains a sterile alpha motif (SAM) domain (aa 541-607) and is subject to multiple alternative splicings. It acts as a sequence specific DNA binding transcriptional activator or repressor. It binds to DNA as a homotetramer. Its DNA binding region is localized to amino acids 170-362. It has a transcription activation (TA) region that is localized in amino acids 1-107 and a transactivation inhibition region that resides in amino acids 610-680, which can interact with and inhibit the activity of the N-terminal TA domain of TA-type isoforms. Twelve different isoforms of TP63 have been described that are produced by alternative promoter usage and alternative splicing. These isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. The ratio of DeltaN-type (those lacking the TA domain) and TA-type isoforms governs the maintenance of epithelial stem cell compartments and regulates the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Isoform 2 is reported to activate RIPK4 transcription and may be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ghioni, p., et al. (2002). Mol. Cell Biol. 22(24); 8659-8668).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable