ZRB1709

Anti-XIAP Antibody, clone 3H4 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): E3 ubiquitin-protein ligase XIAP;EC:2.3.2.27;Baculoviral IAP repeat-containing protein 4;IAP-like protein;ILP;hILP;Inhibitor of apoptosis protein 3;IAP-3;hIAP-3;hIAP3;RING-type E3 ubiquitin transferase XIAP;X-linked inhibitor of apoptosis protein;X-linked
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3H4, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 56.69 kDa; observed mol wt ~52 kDa
• purified by: using Protein A
• species reactivity: rat, monkey, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_001158
• UniProt accession no.: P98170
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... XIAP(331)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3H4 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects E3 ubiquitin-protein ligase XIAP. It targets an epitope within 24 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of human XIAP.

Application

Quality Control Testing

Evaluated by Western Blotting in COS-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected XIAP in COS-7 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected XIAP in C6 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected XIAP in A431 and HepG2 cells..

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected XIAP in human kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound XIAP peptide with a KD of 5.0 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in COS-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected XIAP in COS-7 cell lysate.

Target description

E3 ubiquitin-protein ligase XIAP (UniProt: P98170; also known as EC:2.3.2.27, Baculoviral IAP repeat-containing protein 4, IAP-like protein, ILP, hILP, Inhibitor of apoptosis protein 3, IAP-3, hIAP-3, hIAP3, RING-type E3 ubiquitin transferase XIAP, X-linked inhibitor of apoptosis protein, X-linked IAP) is encoded by the XIAP (also known as API3, BIRC4, IAP3) gene (Gene ID: 331) in human. XIAP is one of the most potent anti-apoptotic IAP family member that contains three BIR domains (aa 26-93; 163-230; and 265-330). It is reported to directly neutralize caspase-9 via its BIR3 domain and the effector caspases-3 and -7 via its BIR2 domain. The BIR3 domain interacts with the Apaf-1/caspase-9 holoenzyme by sequestering the N-terminus of the small subunit of processed caspase-9. In addition to its role in regulating caspases and apoptosis, it also modulates inflammatory signaling and immunity, mitogenic kinase signaling, cell proliferation, and cell invasion and metastasis. It can directly bind to the active site pocket of caspase 3 and 7 and obstructs substrate entry. Aberrant expression of XIAP is reported in a variety of human cancers and it mediates cellular resistance to chemotherapeutic drugs. XIAP also acts as an E3 ubiquitin-protein ligase and regulates NF- B signaling. Other target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, MEKK2, DIABLO/SMAC, and Survivin. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Abbas, R., and Larisch, S. (2020). Cells. 9(3); 663; Obexer, P., and Ausserlechner, MJ. (2014). Front. Oncol. 4; 197).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable