ZRB1634

Anti-CD147 Antibody, clone 1J7 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Basigin;5F7;Collagenase stimulatory factor;Extracellular matrix metalloproteinase inducer;EMMPRIN;Hepatoma-associated antigen;HAb18G;Leukocyte activation antigen M6;OK blood group antigen;Tumor cell-derived collagenase stimulatory factor;TCSF;CD147
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1J7, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 42.2 kDa; observed mol wt ~34 to 52 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): bovine
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Internal
• Protein ID accession no.: NP_001719
• UniProt accession no.: P35613
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... BSG(682)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1J7 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects CD147 (EMMPRIN). It targets an epitope within 20 amino acids from the internal region. It is predicted to react with isoforms 1 and 2.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the internal region of human CD147.

Application

Quality Control Testing

Evaluated by Western Blotting in HEK293T cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CD147 in HEK293T cell lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CD147 in HeLa cell lysate.

Flow Cytometry Analysis: 1 μg from a representative lot detected CD147 in one million HeLa cells and Human peripheral blood mononuclear cells (PBMC).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD147 in HeLa cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CD147 in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in HEK293T cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CD147 in HEK293T cell lysate.

Target description

Basigin (UniProt: P35613; also known as 5F7, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, EMMPRIN, Hepatoma-associated antigen, HAb18G, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, TCSF, CD147) is encoded by the BSG (also known as UNQ6505/PRO21383) gene (Gene ID: 682) in human. CD147 is a single-pass type I membrane, homooligomeric, glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce the mature form. Four isoforms of CD147 have been described that are produced by alternative promoter usage and alternative splicing. Isoform 1 is reported to be essential for normal retinal development and maturation. It is expressed retinal cone photoreceptors. In association with GLUT1 glucose transporter, it promotes retinal cone survival by enhancing aerobic glycolysis and accelerates the entry of glucose into photoreceptors. Isoform 2 is expressed in erythrocytes, heart, kidney, skeletal muscle, and testis. It serves as a signaling receptor for cyclophilins and is essential for cyclophilin A and B-dependent signaling related to chemotaxis and adhesion of immune cells. It also serves as a coreceptor for VEGFR2 in endothelial cells and enhances VEGFA-mediated activation and downstream signaling. CD147 expression has been reported in several human tumors where it plays a key role in tumor progression by stimulating the secretion of matrix metalloproteinases and cytokines. It regulates cell proliferation, inhibits apoptosis, enhances tumor cell migration, metastasis, and differentiation, especially under hypoxic conditions. Clone 1J7 is expected to react with isoforms 1 and2. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ali, NA., et al., (2015). Cell. 161(4); 817-832; Khayati, F., et al. (2015). Oncotarget. 6(12); 9766-9780; Xiong, L., et al. (2014). Int. J. Mol. Sci. 15(10); 17411-17441; Riethdorf, S., et al. (2006). Int. J. Cancer. 119(8); 1800-1810).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable