ZRB1483

Anti-GADD153/DDIT3 Antibody, clone 3I18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): DNA damage-inducible transcript 3 protein;DDIT-3;C/EBP zeta;C/EBP-homologous protein;CHOP;C/EBP-homologous protein 10;CHOP-10;CCAAT/enhancer-binding protein homologous protein;Growth arrest and DNA damage-inducible protein GADD153
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3I18, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 19.17 kDa; observed mol wt ~27 kDa
• purified by: using Protein A
• species reactivity: rat, mouse, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): ELISA: suitable; affinity binding assay: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Unknown
• Protein ID accession no.: NP_004074
• UniProt accession no.: P35638
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... DDIT3(1649)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3I18 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects DNA damage-inducible transcript 3 protein (DDIT3/GADD153).

Immunogen

His-tagged, full-length recombinant Human DNA damage-inducible transcript 3 protein (DDIT3/GADD153).

Application

Quality Control Testing

Evaluated by Western Blotting in NIH3T3 cells nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GADD153/DDIT3 in NIH3T3 cells nuclear extract.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GADD153/DDIT3 in lysate from C6 cells and in HeLa cells treated with Tunicamycin (5 g/mL; 6 h).

Enzyme Immunoassay Analysis (ELISA): Serial dilutions from a representative lot detected recombinant Human GADD153/DDIT3.

Affinity Binding Assay: A representative lot of this antibody bound GADD153 DDIT3 with a KD of 7.2 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in NIH3T3 cells nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GADD153/DDIT3 in NIH3T3 cells nuclear extract.

Target description

DNA damage-inducible transcript 3 protein (UniProt: P35638; also known as DDIT-3, C/EBP zeta, C/EBP-homologous protein, CHOP, C/EBP-homologous protein 10, CHOP-10, CCAAT/enhancer-binding protein homologous protein, Growth arrest and DNA damage-inducible protein GADD153) is encoded by the DDIT3 (also known as CHOP, CHOP10, GADD153) gene (Gene ID: 1649) in human. GADD153 is a multi-functional transcription factor that participates in endoplasmic reticulum (ER) stress response and induces cell cycle arrest and apoptosis in response to ER stress. It is localized in the cytoplasm under non-stressed conditions and translocates to nucleus under following ER stress. Its N-terminal region contains the transcriptional activation domain, and the C-terminal has basic-leucine zipper (bZIP) domain consisting of a basic amino-acid-rich DNA-binding region followed by a leucine zipper dimerization motif. It also contains two adjacent serine residues (79 and 82) that can serve as substrates of the p38 MAP kinase family. It is phosphorylated at serine residues by MAPK14, which enhances its transcriptional activation. However, phosphorylation of serine residues by casein kinase 2 (CK2) is reported to inhibit its transcriptional activation activity. GADD153 plays a regulatory role in the inflammatory response through the induction of caspase-11, which can induce the activation of caspase-1 (CASP1) leading to the activation of pro-IL-1β to mature IL-1β. It is reported to positively regulate the transcription of TRIB3, IL6, IL8, IL23, DR5, GADD34, PUMA, BIM, and ERO1L and negatively regulates the expression of Bcl-2, MYOD1. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ghosh, AP., et al. (2012). PLoS One. 7(6); e39586; Oyadomari, S., and Mori, M. (2004). Cell Death Differ. 11(4); 381-389).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable