ZRB1419
Anti-phospho-eIF-2α-Ser51 Antibody, clone 4N22 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
About This Item
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
4N22, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 36.11 kDa
observed mol wt ~36 kDa
purified by
using Protein A
species reactivity
human
species reactivity (predicted by homology)
bovine, rat
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
phosphorylation (pSer51)
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in lysate from HeLa cells treated with Calyculin A.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected phospho-eIF-2a -Ser51 in HeLa cells treated with Calyculin A (50 nM; 30 min).
Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-eIF-2a -Ser51 in human prostate cancer tissue sections.
Peptide Inhibition Assay: Target band detection in lysate from HeLa cells treated with Calyculin A (50 nM; 30 min) was prevented by preblocking of a representative lot with phosphorylated eIF-2a -Ser51, but not with the corresponding non-phosphopeptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-eIF-2a -Ser51 in HeLa cells treated with Calyculin A.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
Disclaimer
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
ZRB1419-4X25UL:
ZRB1419-25UL:
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service