ZRB1346

Anti-Golgin-97 Antibody, clone 4K16, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Golgin subfamily A member 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit (recombinant)
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4K16, monoclonal; recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 88.18 kDa; observed mol wt ~88 kDa
• species reactivity: human
• species reactivity (predicted by homology): rat
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: Q92805
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... GOLGA1(2800)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 4K16 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Golgin-97. It targets an epitope within 20 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Gogin-97.

Application

Anti-Golgin-97, clone 4K16 ZooMAb, Cat. No. ZRB1346, is a recombinant Rabbit monoclonal antibody that specifically targets Golgin-97 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin) and Western Blotting.

Immunocytochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected Golgin-97 in A431, HUVEC, and HepG2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Golgin-97 in human testis tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Golgin subfamily A member 1 (UniProt: Q92805; also known as Golgin-97) is encoded by the GOLGA1 gene (Gene ID: 2800) in human. Golgin-97 is a peripheral membrane protein that is involved in vesicular trafficking at the Golgi apparatus level. It participates in endosome-to-Golgi trafficking. Golgin-97 is shown to be a binding partner for potassium channel Kir2.1 and is essential for targeting this channel to trans-Golgi network. The GRIP domain (aa 688-737) of Golgin-97 associates with the cytoplasmic domain of Kir2.1. Antibodies against Golgin-97 are found in sera of patients with Sjoegren syndrome characterized by dry mouth and dry eyes. During viral infection, Golgin-97 is reportedly transported to the sites of virus replication and assembly and becomes incorporated into the virions where it associates with the insoluble core protein fraction. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Alzhanova, D., and Hruby, DE. (2006). J. Virol. 80(23); 11520-11527; Taneja, TK et al. (2018). Front Physiol. 9; 1070).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable