ZRB1314

Anti-LONP1 Antibody, clone 1I18, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): EC:3.4.21.53, LONHs, LONP, Lon protease homolog mitochondrial, Lon protease-like protein, Mitochondrial ATP-dependent protease Lon, Serine protease 15
• UNSPSC Code: 12352203
• NACRES: NA.84

Properties

• biological source: rabbit (recombinant)
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1I18, monoclonal; recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 106.49 kDa; observed mol wt ~106 kDa
• species reactivity: rat, mouse, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: P36776
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... LONP1(9361)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1I18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Mitochondrial ATP-dependent protease Lon (LONP).

Immunogen

GST/His-tagged recombinant fragment corresponding to 149 amino acids from the central region of Human LONP1.

Application

Anti-LONP1, clone 1I18 ZooMAb, Cat. No. ZRB1314, is a recombinant rabbit monoclonal antibody that specifically detects mitochondrial Lon protease homolog (LONP1) and has been tested for use in Immunocytochemistry, and Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LONP1 in A431, HEK293, and L6 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected LONP1 in human small intestine and rat colon tissue sections.

Immunocytochemistry Analysis: A 1:10-100 dilution from a representative lot detected LONP1 in HeLa, A431, HUVEC, NIH 3T3 and MCF-7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Lon protease homolog, mitochondrial (UniProt: P36776; also known as EC:3.4.21.53, LONHs, Lon protease-like protein, LONP, Mitochondrial ATP-dependent protease Lon, Serine protease 15) is encoded by the LONP1 (also known as PRSS15) gene (Gene ID: 9361) in human. LONP is a homohexameric, nuclear encoded, mitochondrial matrix protein that acts as an ATP-dependent serine protease. It is expressed ubiquitously and is present at high levels in the liver, brain, heart, skeletal muscle, placenta. It is synthesized with a transit peptide (aa 1-67) that helps in its import into the matrix where it is cleaved off to generate the mature form. It is organized in a ring with a central cavity and the ATP-binding and proteolytic domains (AP-domain) form a hexameric chamber. Its N-terminal domain (aa 124-368) is crucial for the overall structure of the protein, maintaining it in a conformation allowing its proper functioning. It is highly conserved throughout evolution and is shown to be crucial for the maintenance of mitochondrial homeostasis. LONP mediates the selective degradation of mutant and abnormal proteins in mitochondria. It also has a chaperone function in the assembly of inner membrane protein complexes. It also participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial DNA. Its DNA and RNA binding is stimulated by substrate and inhibited by ATP binding. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pinti, M., et al. (2016). Biochim. Biophys. Acta 1857(8); 1300-1306).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable