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ZMS1211

Sigma-Aldrich

Anti-Fibrin Antibody, clone 59D8 ZooMAb® Mouse Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):

Fibrinogen beta chain

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

recombinant monoclonal

description

clone 59D8
recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 55.93 kDa
observed mol wt ~56 kDa

purified by

using protein G

species reactivity

mouse, human

packaging

antibody small pack of 25

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

epitope sequence

N-terminal

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... FGB(2244)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 59D8 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Fibrin. It targets an epitope within 7 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 7 amino acids from the N-terminal region of human Fibrinogen β chain.

Application

Quality Control Testing

Evaluated by Western Blotting in Human plasma.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Fibrin in Human plasma.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Fibrin in lysates from HepG2 cells and from Mouse liver tissue..

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Fibrin in Human placenta tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fibrin in Human kidney and Human placenta tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Fibrinogen beta chain (UniProt: P02675) is encoded by the FGB gene (Gene ID: 2244) in human. Fibrinogen beta chain is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to product the mature protein that is further cleaved by thrombin to produce fibrinopeptide B (aa 31-44) and fibrinogen beta chain (45-491). Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. Fibrin monomers spontaneously aggregate into an insoluble gel, which is then covalently stabilized by Factor XIIIa. Fibrin is shown to retain about 98% of the original covalent structure of fibrinogen. Fibrin clot can be broken down by plasmin by cleave between residues 152-153 and 163-164. A mouse monoclonal version of this clone binds to fibrin even in the presence of human fibrinogen at the concentration found in plasma. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonal. (Ref.: Litvinov, RI., and Weisel, JW (2016). Semin. Thromb. Hemost. 42(4); 333-343; Hui, KY., et al. (1983). Science. 222 (4628); 1129-1132).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ZMS1211-4X25UL:
ZMS1211-25UL:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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