ZMS1196

Anti-GFAP Antibody, clone GA5 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Anti-V5 Tag Antibody, clone 1K20-L2 ZooMAb^® Rabbit Monoclonal, Glial fibrillary acidic protein
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: GA5, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 53.63 kDa; observed mol wt ~52 kDa
• purified by: using protein G
• species reactivity: mouse, rat
• species reactivity (predicted by homology): rabbit, porcine, human, chicken
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: C-terminal
• Protein ID accession no.: XP_005668766.1
• UniProt accession no.: F1RR02
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: Porcine ... GFAP(396562)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone GA5 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Glial fibrillary acidic protein (GFAP). It targets an epitope within the C-terminal region.

Immunogen

Purified Glial Fibrillary Acidic Protein (GFAP) from pig spinal cord.

Application

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GFAP in Rat brain tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GFAP in Mouse brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GFAP in Rat brain tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GFAP in Rat brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Glial fibrillary acidic protein (UniProt: F1RR02; also known as GFAP) is encoded by the GFAP gene (Gene ID: 396562) in porcine species. GFAP is a class-III intermediate filament, monomeric, protein that serves as a cell-specific marker that can distinguish astrocytes from other glial cells and is considered as a good marker of astroglial injury where its levels are constantly upregulated. It is not detected outside the central nervous system. However, in traumatic brain injury, blood GFAP levels have been correlated with clinical severity and extent of intracranial pathology. Its levels are also shown to be upregulated with age in rodents and humans. Hence, it can serve as a surrogate marker of aging in the brain. GFAP has a relatively slow metabolic turnover rate, which is consistent with its structural role. It is composed of three distinct regions: an N-terminal head region, a central ROD region, and a C-terminal tail region. The ROD regions are highly conserved among intermediate

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1