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Z2126

Sigma-Aldrich

ZAP-70 Tyrosine Kinase human

≥90% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells (Histidine tagged), buffered aqueous glycerol solution, ≥150 U/mg

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About This Item

MDL number:
MFCD03459122
UNSPSC Code:
12352200
NACRES:
NA.32

recombinant

expressed in baculovirus infected insect cells (Histidine tagged)

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥150 U/mg

mol wt

70 kDa

UniProt accession no.

P43403

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... ZAP70(7535)

Application

Human ZAP-70 Tyrosine Kinase was used for mapping the phosphorylation sites on LAT (linker for activation of T cells) for investigating the mechanism of activation of signalling proteins in T cells.

Biochem/physiol Actions

ZAP-70 Tyrosine Kinase is a protein belongs to Syk family and is essential for T cell activation. It facilitates the upregulation of Fas ligand in activation-induced T cell apoptosis. Additionally, it also plays a pivotal role in LFA-1-dependent T Cell Migration.
ZAP-70 is protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells. It is required for T cell activation. It is activated by Lck-mediated phosphorylation of its tyrosine residues.

Unit Definition

One unit will phosphorylate 1 nmol polyglutamic acid:tyrosine per min at 37 °C at pH 7.0.

Physical form

Solution in 25 mM HEPES, pH 7.5, containing 150 mM NaCl, 0.5 mM DTT, and 50% glycerol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Cartagena Act

Cartagena Act Listed

JAN Code

Z2126-20UG:
Z2126-VAR:
Z2126-20UG-PW:
Z2126-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C M Eischen et al.
Journal of immunology (Baltimore, Md. : 1950), 159(3), 1135-1139 (1997-08-01)
Activation-induced cell death (AICD) is initiated by the TCR-dependent up-regulation of Fas ligand (FasL) mRNA. The subsequently generated soluble or cell-associated FasL gene products bind Fas, leading to apoptosis of the T cells. Although TCR stimulation is essential to initiate
P E Paz et al.
The Biochemical journal, 356(Pt 2), 461-471 (2001-05-23)
T-cell-receptor (TCR)-mediated LAT (linker for activation of T cells) phosphorylation is critical for the membrane recruitment of signalling complexes required for T-cell activation. Although tyrosine phosphorylation of LAT is required for recruitment and activation of signalling proteins, the molecular mechanism
R D Soede et al.
The Journal of cell biology, 142(5), 1371-1379 (1998-09-10)
The ZAP-70 tyrosine kinase is essential for T cell activation by the T cell receptor. We show that ZAP-70 is also required for migration of T cells that is dependent on the integrin LFA-1. Invasion of TAM2D2 T cell hybridoma
D H Chu et al.
Immunological reviews, 165, 167-180 (1998-12-16)
The processes of T-cell development and activation employ similar immature and mature receptors as well as similar signal transduction pathways to achieve different outcomes. Many signaling molecules are shared between the receptor signaling pathways, including two families of cytoplasmic protein

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