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Key Documents

Safety Information

WH0002194M1

Sigma-Aldrich

Monoclonal Anti-FASN antibody produced in mouse

clone 3F2-1F3, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-FAS, Anti-MGC14367, Anti-MGC15706, Anti-OA519, Anti-fatty acid synthase

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About This Item

MDL number:
MFCD02262888
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3F2-1F3, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: suitable
western blot: 1-5 μg/mL

isotype

IgG1κ

GenBank accession no.

BC007909

UniProt accession no.

P49327

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FASN(2194)

General description

FASN (fatty acid synthase) encodes a fatty acid synthase (FAS) enzyme. This gene is located on human chromosome 17.
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. (provided by RefSeq)

Immunogen

FASN (AAH07909, 1 a.a. ~ 439 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
MSTNDTIVSGTLPQRMASCLEVLDLFLNQPHMVLSSFVLAEKAAAYRDRDSQRDLVEAVAHILGIRDLAAVNLDSSLADLGLDSLMSVEVRQTLERELNLVLSVREVRQLTLRKLQELSSKADEASELACPTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASGLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG

Biochem/physiol Actions

FASN (fatty acid synthase) is essential for de novo fatty-acid synthesis. It plays a major role in glioma neovascularization. The product of this gene helps in the production of fatty acids from acetyl-CoA and malonyl-CoA. Absence of FASN may block migration, invasion and healing of hepatoma carcinoma cells by reducing HIF-1α (hypoxia-inducible factor 1-α) and IGFBP1 (insulin-like growth factor binding protein-1).

Physical form

Solution in phosphate buffered saline, pH 7.4

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

WH0002194M1-50UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effects of fatty acid synthase inhibitors on lymphatic vessels: an in vitro and in vivo study in a melanoma model
Bastos DC, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology (2017)
FASN, dietary fat intake, and risk of uterine leiomyomata in the Black Women's Health Study
Wise LA, et al.
Fertility and Sterility, 106(5), 1136-1141 (2016)
Inhibition of FASN suppresses migration, invasion and growth in hepatoma carcinoma cells by deregulating the HIF-1?/IGFBP1 pathway
Gong J, et al.
International Journal of Oncology, 50(3), 883-892 (2017)
Inhibition of fatty acid synthase suppresses neovascularization via regulating the expression of VEGF-A in glioma
Zhou Y, et al.
Journal of Cancer Research and Clinical Oncology, 142(12), 2447-2459 (2016)
Lakshmi Reddy Bollu et al.
Cell cycle (Georgetown, Tex.), 13(15), 2415-2430 (2014-12-09)
Increased expressions of fatty acid synthase (FASN) and epidermal growth factor receptor (EGFR) are common in cancer cells. De novo synthesis of palmitate by FASN is critical for the survival of cancer cells via mechanisms independent of its role as

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