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Safety Information

WH0001656M1

Sigma-Aldrich

Monoclonal Anti-DDX6 antibody produced in mouse

clone 3D2, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, Anti-HLR2, Anti-P54, Anti-RCK

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3D2, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
western blot: 1-5 μg/mL

isotype

IgG1κ

GenBank accession no.

BC065007

UniProt accession no.

P26196

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DDX6(1656)

General description

DEAD-box helicase 6 (DDX6), also known as RCK/p54, is encoded by the gene mapped to human chromosome 11q23.3. The encoded protein belongs to the DEAD-box RNA helicase family.
This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. (provided by RefSeq)

Immunogen

DDX6 (AAH65007, 1 a.a. ~ 483 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP

Biochem/physiol Actions

DEAD-box helicase 6 (DDX6) is a key constituent of messenger ribonucleoprotein (mRNP) complexes. It facilitates the translation of mRNA in meiotic cells. DDX6 regulates adult progenitor cell function through differentiation-inducing transcripts and by promoting the translation of self-renewal and proliferation mRNAs. Deletion of the gene leads to premature differentiation and decreased proliferation of epidermal progenitor cells. Elevated expression of the gene is associated with the development of colorectal tumors.

Physical form

Solution in phosphate buffered saline, pH 7.4

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

WH0001656M1-100UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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RNA helicase p54 (DDX6) is a shuttling protein involved in nuclear assembly of stored mRNP particles
Smillie DA and Sommerville J
Journal of Cell Science, 115, 395-407 (2002)
Cryptic ins(2;11) with clonal evolution showing amplification of 11q23?q25 either on hsr(11) or on dmin, in a patient with AML-M2
Vazquez I, et al.
Leukemia, 18, 2041-2044 (2004)
DDX6 Orchestrates Mammalian Progenitor Function through the mRNA Degradation and Translation Pathways.
Wang Y, et al.
Molecular Cell, 60, 118-130 (2015)
Rck/p54 interacts with APP mRNA as part of a multi-protein complex and enhances APP mRNA and protein expression in neuronal cell lines.
Broytman, O., et al.
Neurobiology of Aging (2008)
Overexpression of rck/p54, a DEAD box protein, in human colorectal tumours.
Nakagawa Y, et al.
British Journal of Cancer, 80, 914-917 (1999)
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