Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

Safety Information

W3269

Sigma-Aldrich

Monoclonal Anti-WDR62 antibody produced in mouse

~1.0 mg/mL, clone 3G8, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-C19orf14, Anti-WD repeat domain 62

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3G8, monoclonal

form

buffered aqueous solution

mol wt

antigen ~170 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 0.5-1 μg/mL using HEK-293T cells over expressing human WDR62
western blot: 0.5-1 μg/mL using HEK-293T cells over expressing human WDR62

isotype

IgG2a

UniProt accession no.

O43379

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... WDR62(284403)

General description

Monoclonal Anti-WDR62 (mouse IgG2a isotype) is derived from the hybridoma 3G8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein encoding a fragment of human WDR62. WD repeat 62 is a member of WD-repeats and contains 15 WD repeats. WD-repeats consists of minimally conserved domains of 40-60 amino acids, which are initiated by glycine-histidine (GH) dipeptide 11 to 24 residues from the N-terminus and ends with tryptophan-aspartic acid (WD) dipeptide at the C terminus.

Application

Monoclonal Anti-WDR62 antibody produced in mouse has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

WD repeat 62 (WDR62) is identified in a phosphoproteome analysis as a protein associated with the mitotic spindle. It physically interacts with RNA-binding protein (RALY), TATA-binding protein (TBP), C1 or f103, mitogen-activated protein kinase (MAPK9) and itself. WDR62 potentiates c-Jun N-terminal kinase (JNK) activity. Mutations in this gene are linked to microcephaly and cortical malformations.

Target description

WDR62 contains 15 WD repeats and has been identified in aphosphoproteome analysis as a protein associated with the mitotic spindle. WD40 proteins are implicated in many essential biologicalfunctions including adaptor/regulatory modules in signaltran

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

W3269-BULK:
W3269-VAR:
W3269-25UL:
W3269-200UL:

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
106M4788
089K4759

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Mutations in WDR62, encoding a centrosome-associated protein, cause microcephaly with simplified gyri and abnormal cortical architecture
Timothy WY, et al.
Nature Genetics, 42(11), 1015-1015 (2010)
Identification and analysis of a novel dimerization domain shared by various members of c-Jun N-terminal kinase (JNK) scaffold proteins
Cohen-Katsenelson K, et al.
The Journal of Biological Chemistry, 288(10), 7294-7304 (2013)
Marie A Bogoyevitch et al.
Journal of cell science, 125(Pt 21), 5096-5109 (2012-08-18)
The impact of aberrant centrosomes and/or spindles on asymmetric cell division in embryonic development indicates the tight regulation of bipolar spindle formation and positioning that is required for mitotic progression and cell fate determination. WD40-repeat protein 62 (WDR62) was recently
D Li et al.
Cellular and molecular life sciences : CMLS, 58(14), 2085-2097 (2002-01-30)
Defined by the presence of four or more repeating units containing a conserved core of approximately 40 amino acids that usually ending with tryptophan-aspartic acid (WD), WD-repeat proteins belong to a large and fast-expanding conservative protein family. As demonstrated by
Ksenya Cohen-Katsenelson et al.
The Journal of biological chemistry, 288(10), 7294-7304 (2013-01-24)
Mitogen-activated protein kinases (MAPKs) form a kinase tier module in which MAPK, MAP2K, and MAP3K are held by scaffold proteins. The scaffold proteins serve as a protein platform for selective and spatial kinase activation. The precise mechanism by which the
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service