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Safety Information

V9505

Sigma-Aldrich

Monoclonal Anti-Vitamin B12 antibody produced in mouse

clone CD-29, ascites fluid

Synonym(s):

Anti-Cyanocobalamin

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About This Item

MDL number:
MFCD00164758
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

CD-29, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:5,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Vitamin B12 antibody reacts with vitamin B12, as well as with vitamin B12-KLH and vitamin B12-BSA conjugates in human plasma and serum.
Vitamin B12 or cobalamin is a water-soluble vitamin. It is the largest and most complex among all the vitamins. Vitamin B12 is naturally found in animal foods, meat, milk, egg, fish and shellfish.

Application

Monoclonal Anti-Vitamin (B12) antibody has been used in enzyme linked immunosorbent assay (ELISA)
Monoclonal Anti-Vitamin B12 antibody produced in mouse has also been used in immunocytochemistry.

Biochem/physiol Actions

Vitamin B12 serves as a cofactor for methionine synthase and L-methylmalonyl−coenzyme A mutase. It is essential for the maintenance and development of CNS. Vitamin B12 deficiency leads to megaloblastic anemia and neuropathy.
Vitamin B12 regulates genomic stability. While increased levels of vitamin B12 have been associated with hepatic and hematological disorders; cobalamin deficiency has been associated with orthostatic hypotension and depression . The antibody also detects coenzyme B12 and methyl vitamin B12, but does not associate with BSA, folic acid, and 5-methyltetrahyrofolic acid.
Vitamin B12 and folic acid can be used for treating disorders of central nervous system (CNS) development, mood, and cognitive decline, including some dementias.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

V9505-.5ML:
V9505-.2ML:
V9505-VAR:
V9505-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Fenech
Mutation research, 475(1-2), 57-67 (2001-04-11)
Folic acid plays a critical role in the prevention of chromosome breakage and hypomethylation of DNA. This activity is compromised when Vitamin B12 (B12) concentration is low because methionine synthase activity is reduced, lowering the concentration of S-adenosyl methionine (SAM)
Vitamin B12, folic acid, and the nervous system
Reynolds E
Lancet Neurology, 5(11), 949-960 (2006)
Allan Moore et al.
Clinical autonomic research : official journal of the Clinical Autonomic Research Society, 14(2), 67-71 (2004-04-20)
Orthostatic hypotension (OH) and vitamin B12 deficiency are common disorders in older people. Several case series have reported an association between vitamin B12 deficiency and OH. The effect of vitamin B12 replacement on this dysfunction has not been studied. We
A A M Ermens et al.
Clinical biochemistry, 36(8), 585-590 (2003-11-26)
Elevated levels of serum cobalamin may be a sign of a serious, even life-threatening, disease. Hematologic disorders like chronic myelogeneous leukemia, promyelocytic leukemia, polycythemia vera and also the hypereosinophilic syndrome can result in elevated levels of cobalamin. Not surprisingly, a
Alec Coppen et al.
Journal of psychopharmacology (Oxford, England), 19(1), 59-65 (2005-01-27)
We review the findings in major depression of a low plasma and particularly red cell folate, but also of low vitamin B12 status. Both low folate and low vitamin B12 status have been found in studies of depressive patients, and

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