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V7754

Sigma-Aldrich

V5 Peptide

≥97% (HPLC), lyophilized powder

Synonym(s):

V5 Tag Peptide

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About This Item

Empirical Formula (Hill Notation):
C67H113N17O21S
CAS Number:
922704-46-9
Molecular Weight:
1524.78
MDL number:
MFCD03458947
UNSPSC Code:
12352204
NACRES:
NA.56

product name

V5 Peptide, ≥97% (HPLC), lyophilized powder

Quality Level

200

Assay

≥97% (HPLC)

form

lyophilized powder

mol wt

calculated mol wt 1524.78 Da

storage temp.

−20°C

Amino Acid Sequence

Cys-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr

General description

V5 Tag Peptide is a synthetic peptide that has an amino acid sequence correspondinh to amino acids 95-108 of the non-structural protein V and to the RNA polymerase a subunit (P protein), of Paramyxovirus SV5 with an N-terminal cysteine.

Application

The peptide is useful for displacement of V5-tagged fusion proteins from anti-V5 antibody in immunoassays. The successful inhibition of antibody binding of V5 peptide demonstrates binding is specific.

Other Notes

The amino acid sequence corresponds to amino acids 95-109 of non-structural protein V and to RNA polymerase α subunit (P protein), of Paramyxovirus SV5 with an N-terminal cysteine.
Amino acid sequence is N-Cys-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-C
Lyophilized from 0.1% TFA in H2O

Preparation Note

To prepare a stock solution, dissolve in water to a final concentration of 5 mg/mL.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

V7754-25MG-PW:
V7754-4MG:
V7754-4MG-PW:
V7754-BULK:
V7754-VAR:
V7754-25MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yan-ling Zhang et al.
Archives of biochemistry and biophysics, 483(1), 45-54 (2009-01-06)
Tissue specific amplification of glucocorticoid action through NADPH-dependent reduction of inactive glucocorticoid precursors by 11beta-hydroxysteroid dehydrogenase (11beta-HSD1) contributes to the development of visceral obesity, insulin resistance and Type 2 Diabetes. Hexose-6-phosphate dehydrogenase (H6PDH) is believed to supply NADPH for the
Rui Chen et al.
PloS one, 12(12), e0190241-e0190241 (2017-12-28)
Survival of cancer cells in the harsh tumor microenvironment, characterized by oxygen and glucose deprivation, requires rapid initiation of cytoprotective measures. Metabolites whose levels change during stress are ideal signaling cues, particularly if used in post-translational modifications of stress-responsive signal

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