UC9601

UltraClear^™ Sequencing Reaction Clean-Up Kit

pkg of 1 plates, sufficient for 1 96-well plate purifications

• Synonym(s): Sequencing Clean-Up Kit, UltraClear Reaction Kit
• UNSPSC Code: 41105600
• NACRES: NA.52

Properties

• usage: sufficient for 1 96-well plate purifications
• Quality Level: 200
• packaging: pkg of 1 plates
• storage temp.: 15-25°C

Description

General description

The UltraClear Sequencing Reaction Clean-Up Plates offer a rapid and simple method for the clean-up of DNA sequencing reactions in a 96-well format. The plates use ultrafiltration membranes to separate low molecular weight contaminants, such as unincorporated dye terminators, dNTPs, and residual salts from the sequencing reaction products.

Following thermocycling, the sequencing reactions are diluted with Sequencing Solution. This mixture is then filtered through the UltraClear plate by centrifugation. The sequencing reaction products are retained on the surface of the ultrafiltration membrane while the lower molecular weight contaminants pass through the membrane and are collected as waste. The purified sequencing products are then resuspended in Sequencing Solution and are ready for injection onto capillary-based DNA analyzers.

Features and Benefits

• Effective clean-up of ABI BigDye^® chemistries
• Simple centrifugation-based procedure
• Convenient 96-well format

Other Notes

For additional information, please see www.sigma-aldrich.com/sequencing.

Legal Information

BigDye is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

UltraClear is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable